Document Detail


Tea catechins protect against lead-induced ROS formation, mitochondrial dysfunction, and calcium dysregulation in PC12 cells.
MedLine Citation:
PMID:  12971804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies have shown that lead causes oxidative stress by inducing the generation of reactive oxygen species (ROS) and reducing the antioxidant defense system of cells, which suggests that antioxidants may play an important role in the treatment of lead poisoning. The present study was designed to elucidate whether tea catechins had any protective effects on altered oxidative stress parameter in PC12 cells exposed to lead. The experimental results showed that lead decreased PC12 cell viability and induced a rapid elevation of [Ca(2+)](i), which was followed by an accumulation of ROS and a decrease of mitochondrial membrane potential (MMP). Treatment by tea catechins significantly increased cell viability, decreased intracellular Ca(2+) levels and ROS formation, and improved MMP in PC12 cells exposed to lead. The galloylated catechins showed a greater effect on ROS formation and mitochondrial dysfunction than that of nongalloylated catechins, which was similar to the result of their scavenging ability on free radical. In view of the time course of ROS formation and mitochondrial dysfunction and their correlation, our results also suggested that the beneficial effects of tea catechins on MMP are related, at least in part, to its ability to scavenge ROS in PC12 cells exposed to 100 microM Pb(2+). The present results suggest that tea catechins supplementation may play a role for modulating oxidative stress in PC12 cells exposed to lead.
Authors:
Liuji Chen; Xianqiang Yang; Hongli Jiao; Baolu Zhao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemical research in toxicology     Volume:  16     ISSN:  0893-228X     ISO Abbreviation:  Chem. Res. Toxicol.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-15     Completed Date:  2004-07-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8807448     Medline TA:  Chem Res Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1155-61     Citation Subset:  IM    
Affiliation:
National Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, Shanghai, China 200032.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism*
Catechin / analogs & derivatives*,  chemistry*,  pharmacology*,  physiology
Cell Death / drug effects,  physiology
Cell Survival / drug effects,  physiology
Dose-Response Relationship, Drug
Lead / adverse effects*,  antagonists & inhibitors*
Membrane Potentials / drug effects
Mitochondria / drug effects*,  pathology
Oxidative Stress / drug effects
PC12 Cells
Rats
Reactive Oxygen Species / chemistry,  metabolism*
Tea / chemistry*
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species; 0/Tea; 0/gallocatechin gallate; 154-23-4/Catechin; 7439-92-1/Lead; 7440-70-2/Calcium; 863-03-6/epicatechin gallate; 989-51-5/epigallocatechin gallate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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