| Tea catechin auto-oxidation dimers are accumulated and retained by Caco-2 human intestinal cells. | |
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MedLine Citation:
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PMID: 20579525 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Despite the presence of bioactive catechin B-ring auto-oxidation dimers in tea, little is known regarding their absorption in humans. Our hypothesis for this research is that catechin auto-oxidation dimers are present in teas and are absorbable by human intestinal epithelial cells. Dimers (theasinensins [THSNs] and P-2 analogs) were quantified in commercial teas by high-performance liquid chromatography-mass spectrometry. (-)-Epigallocatechin (EGC) and (-)-epigallocatechin gallate (EGCG) homodimers were present at 10 to 43 and 0 to 62 mumol/g leaf, respectively. The EGC-EGCG heterodimers were present at 0 to 79 mumol/g. The potential intestinal absorption of these dimers was assessed using Caco-2 intestinal cells. Catechin monomers and dimers were detected in cells exposed to media containing monomers and preformed dimers. Accumulation of dimers was significantly greater than monomers from test media. Three-hour accumulation of EGC and EGCG was 0.19% to 0.55% and 1.24% to 1.35%, respectively. Comparatively, 3-hour accumulation of the EGC P-2 analog and THSNs C/E was 0.89% +/- 0.28% and 1.53% +/- 0.36%, respectively. Accumulation of P-2 and THSNs A/D was 6.93% +/- 2.1% and 10.1% +/- 3.6%, respectively. The EGCG-EGC heterodimer P-2 analog and THSN B 3-hour accumulation was 4.87% +/- 2.2% and 4.65% +/- 2.8%, respectively. One-hour retention of P-2 and THSNs A/D was 171% +/- 22% and 29.6% +/- 9.3% of accumulated amount, respectively, suggesting intracellular oxidative conversion of THSNs to P-2. These data suggest that catechin dimers present in the gut lumen may be readily absorbed by intestinal epithelium. |
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Authors:
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Andrew P Neilson; Brian J Song; Teryn N Sapper; Joshua A Bomser; Mario G Ferruzzi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Nutrition research (New York, N.Y.) Volume: 30 ISSN: 1879-0739 ISO Abbreviation: Nutr Res Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-06-28 Completed Date: 2010-10-26 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 8303331 Medline TA: Nutr Res Country: United States |
Other Details:
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Languages: eng Pagination: 327-40 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Food Science, Purdue University, West Lafayette, IN 47907, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Benzopyrans
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chemistry,
metabolism Caco-2 Cells Camellia sinensis / chemistry* Catechin / analogs & derivatives, chemistry, metabolism* Chromatography, Liquid Dimerization Epithelial Cells / metabolism* Humans Intestinal Absorption Intestinal Mucosa / metabolism* Mass Spectrometry Oxidation-Reduction Phenols / chemistry, metabolism Polymers / chemistry, metabolism Tea / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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CA119210-01A1/CA/NCI NIH HHS; R03 CA119210-01A1/CA/NCI NIH HHS; R03 CA119210-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Benzopyrans; 0/Phenols; 0/Polymers; 0/Tea; 0/theasinensin A; 154-23-4/Catechin; 69522-59-4/P-2 |
| Comments/Corrections | |
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