| Tc-99m-HL91 imaging in the early detection of neuronal injury in a neonatal rat model of hypoxic ischemia*. | |
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MedLine Citation:
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PMID: 22610194 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: : Hypoxic-ischemic insult in newborns results in progressive neuronal loss. For neuroprotective therapy to be effective, it is important to identify high-risk neonates soon after birth. Tc-labeled imaging agent, Tc-99m-HL91, developed as a putative hypoxic reagent, has been reported to demonstrate increased uptake in ischemic myocardium. We hypothesized that Tc-99m-HL91 is sensitive for the early identification of hypoxic-ischemic injury in neonatal rat brains. DESIGN: : Laboratory investigation. SETTING: : University research laboratory. SUBJECTS: : Sprague-Dawley rat pups. INTERVENTIONS: : Postnatal day-7 pups were divided into four groups: hypoxic-ischemia, hypoxia-only, ischemia-only, and controls. In the early (2 hrs), intermediate (20 hrs), and late (44 hrs) reoxygenation phases, Tc-99m-HL91 in vivo and ex vivo imaging and quantitative autoradiography were performed. Regions of interest were drawn to calculate the contrast ratio of Tc-99m-HL91 uptake between the ipsilateral and contralateral hemispheres. Pathology, cerebral blood flow, and blood-brain barrier damage were determined. MEASUREMENTS AND MAIN RESULTS: : After hypoxic-ischemia, there were very few pyknotic neurons in the early phase, many pyknotic neurons in the intermediate phase, and extensive neuronal loss in the late phase postreoxygenation. Blood-brain barrier damage occurred in the early phase, progressed in the intermediate phase, and became extensive in the late phase. The hypoxia-only and ischemia-only pups showed no neuronal or blood-brain barrier damage and had higher cerebral blood flow postreoxygenation compared with the hypoxia-ischemia pups. Regions of interest analysis of in vivo and ex vivo images and autoradiography revealed significantly higher Tc-99m-HL91 contrast ratio at early and intermediate phases, not late phase of hypoxic-ischemic group. Hypoxic-ischemia group had significantly higher contrast ratio values in the early and intermediate phases than the hypoxia-only and ischemia-only groups. A contrast ratio value of 0.15 in the early phase on postnatal day 7 had a sensitivity of 0.95 and specificity of 0.89 in detecting significant hypoxic-ischemic lesions on postnatal day 21. CONCLUSION: : Tc-99m-HL91 uptake is sensitive for the early detection of hypoxic-ischemic injury in neonatal brains. |
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Authors:
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Bi-Fang Lee; Lan-Wan Wang; Sheng-Hsiang Lin; Ting-Jyun Jhuo; Nan-Tsing Chiu; Chao-Ching Huang; Chien-Chung Hsia; Lie-Hang Shen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Critical care medicine Volume: 40 ISSN: 1530-0293 ISO Abbreviation: Crit. Care Med. Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-05-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0355501 Medline TA: Crit Care Med Country: United States |
Other Details:
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Languages: eng Pagination: 1930-8 Citation Subset: AIM; IM |
Affiliation:
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From the Departments of Nuclear Medicine (BFL, TJJ, NTC) and Pediatrics (CCH), and Institute of Clinical Medicine (LWW, SHL, CCH), National Cheng Kung University, College of Medicine, Tainan, Taiwan; Department of Pediatrics (LWW), Chi Mei Medical Center, Tainan, Taiwan; and Institute of Nuclear Energy (CCH, LHS), Atomic Energy Council, Executive Yuan, Lungtan, Taiwan. |
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