Document Detail


Tbx5 is dispensable for forelimb outgrowth.
MedLine Citation:
PMID:  17138667     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tbx5 is essential for initiation of the forelimb, and its deletion in mice results in the failure of forelimb formation. Misexpression of dominant-negative forms of Tbx5 results in limb truncations, suggesting Tbx5 is also required for forelimb outgrowth. Here we show that Tbx5 is expressed throughout the limb mesenchyme in progenitors of cartilage, tendon and muscle. Using a tamoxifeninducible Cre transgenic line, we map the time frame during which Tbx5 is required for limb development. We show that deletion of Tbx5 subsequent to limb initiation does not impair limb outgrowth. Furthermore, we distinguish two distinct phases of limb development: a Tbx5-dependent limb initiation phase, followed by a Tbx5-independent limb outgrowth phase. In humans, mutations in the T-box transcription factor TBX5 are associated with the dominant disorder Holt-Oram syndrome (HOS), which is characterised by malformations in the forelimb and heart. Our results demonstrate a short temporal requirement for Tbx5 during early limb development, and suggest that the defects found in HOS arise as a result of disrupted TBX5 function during this narrow time window.
Authors:
Peleg Hasson; Joanne Del Buono; Malcolm P O Logan
Related Documents :
7840537 - Hair distribution on the phalanges of the hands in malays.
12102417 - Collagen-binding heat shock protein hsp47 expression during healing of fetal skin wounds.
15880507 - Limb chondrogenesis of the seepage salamander, desmognathus aeneus (amphibia: plethodon...
1526527 - Relationship of serum levels of pro-type i collagen peptide, pro-type iii collagen pept...
17393017 - Haemostatic changes related to fibrin formation and fibrinolysis during the first trime...
12517397 - Production of piglets using intracytoplasmic sperm injection (icsi) with flowcytometric...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-30
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  134     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-13     Completed Date:  2007-03-28     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  85-92     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Apoptosis
Embryo, Mammalian
Embryonic Induction / drug effects
Female
Fluorescent Antibody Technique, Indirect
Forelimb / embryology*
Gene Expression Regulation, Developmental
In Situ Hybridization
Mesoderm / metabolism
Mice
Mice, Transgenic
Models, Biological
Pregnancy
T-Box Domain Proteins / genetics,  metabolism*
Tamoxifen / pharmacology
Time Factors
Grant Support
ID/Acronym/Agency:
MC_U117560477//Medical Research Council
Chemical
Reg. No./Substance:
0/T-Box Domain Proteins; 0/T-box transcription factor 5; 094ZI81Y45/Tamoxifen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Functional redundancy among Nanos proteins and a distinct role of Nanos2 during male germ cell devel...
Next Document:  Using the principle of entropy maximization to infer genetic interaction networks from gene expressi...