Document Detail


Tbx1 is required for second heart field proliferation in zebrafish.
MedLine Citation:
PMID:  23335360     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The mammalian outflow tract (OFT) and primitive right ventricle arise by accretion of newly differentiated cells to the arterial pole of the heart tube from multi-potent progenitor cells of the second heart field (SHF). While mounting evidence suggests that the genetic pathways regulating SHF development are highly conserved in zebrafish, this topic remains an active area of investigation.
RESULTS: Here, we extend previous observations demonstrating that zebrafish tbx1 (van gogh, vgo) mutants show ventricular and OFT defects consistent with a conserved role in SHF-mediated cardiogenesis. Surprisingly, we reveal through double in situ analyses that tbx1 transcripts are excluded from cardiac progenitor cells and differentiated cardiomyocytes, suggesting a non-autonomous role in SHF development. Further, we find that the diminutive ventricle in vgo animals results from a 25% decrease in cardiomyocyte number that occurs subsequent to heart tube stages. Lastly, we report that although SHF progenitors are specified in the absence of Tbx1, they fail to be maintained due to compromised SHF progenitor cell proliferation.
CONCLUSIONS: These studies highlight conservation of Tbx1 function in zebrafish SHF biology.
Authors:
Kathleen Nevis; Pablo Obregon; Conor Walsh; Burcu Guner-Ataman; C Geoffrey Burns; Caroline E Burns
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-03-06
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  242     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-17     Completed Date:  2013-11-14     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  550-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Cell Differentiation / genetics
Cell Proliferation*
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
Heart / embryology*,  physiology
Heart Ventricles / cytology,  embryology
Myocytes, Cardiac / cytology,  metabolism,  physiology
Stem Cells / metabolism,  physiology
T-Box Domain Proteins / genetics,  metabolism,  physiology*
Zebrafish* / embryology,  genetics,  metabolism
Zebrafish Proteins / genetics,  metabolism,  physiology
Grant Support
ID/Acronym/Agency:
5F32HL110627/HL/NHLBI NIH HHS; 5R01HL096816/HL/NHLBI NIH HHS; 5R01HL111179/HL/NHLBI NIH HHS; R01 HL096816/HL/NHLBI NIH HHS; R01 HL111179/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/T-Box Domain Proteins; 0/Zebrafish Proteins; 0/tbr1 protein, zebrafish
Comments/Corrections

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