| Taxonomic identification and use of free and entrapped cells of a new Mycobacterium sp., strain Spyr1 for degradation of polycyclic aromatic hydrocarbons (PAHs). | |
| | |
MedLine Citation:
|
PMID: 19096938 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A polycyclic aromatic hydrocarbon (PAH)-degrading bacterial strain Spyr1 was isolated from Greek creosote polluted soil by an enrichment method using pyrene as sole carbon and energy source. Spyr1 was identified as Mycobacterium sp. based on 16S rDNA analysis and it was capable of degrading pyrene, fluoranthene, fluorene, anthracene, and acenaphthene. The effect of entrapment in glass beads and alginate/starch mixtures on the survival and pyrene degradation ability of Spyr1 cells in liquid suspensions and soil microcosms was tested and compared with that of freely suspended cells. In general, free cells showed higher degradation of pyrene and other PAH than immobilized cells. However, immobilized cells could better tolerate PAH and they maintained their viability and PAH degradation capability for at least 1 year after storage at 4 degrees C. Entrapped cells in glass beads exhibited better pyrene biodegradation performance than alginate/starch entrapped cells in liquid suspensions and could be used effectively for at least ten repeated cycles. Alginate/starch entrapped cells exhibited better yields than glass beads entrapped cells for removing pyrene as well as mixtures of PAH in soil microcosms. |
| | |
Authors:
|
E Karabika; A Kallimanis; A Dados; G Pilidis; C Drainas; A I Koukkou |
Related Documents
:
|
437838 - A mechanism for secretory iga-mediated inhibition of the cell penetration and intracell... 11312708 - Asymmetric ketone reduction with immobilized yeast in hexane: biocatalyst deactivation ... 1107598 - T7 protein synthesis in f-factor-containing cells: evidence for an episomally induced i... 5583988 - Studies on the biosynthesis of gramicidin s in a cell-free system from bacillus brevis.... 6202418 - Sequential expression of mrna-encoded keratin sets in neonatal mouse epidermis: basal c... 1443608 - A quantitative microassay of carbohydrate-mediated cell adhesion to glycoconjugates imm... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-19 |
Journal Detail:
|
Title: Applied biochemistry and biotechnology Volume: 159 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-09-03 Completed Date: 2009-11-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
|
Languages: eng Pagination: 155-67 Citation Subset: IM |
Affiliation:
|
Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, Greece. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biodegradation, Environmental Cells, Immobilized Mycobacterium / classification*, isolation & purification, metabolism* Polycyclic Hydrocarbons, Aromatic / metabolism* Soil Microbiology* Species Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Polycyclic Hydrocarbons, Aromatic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The associations between area of residence, sexual violence victimization, and asthma episodes among...
Next Document: Assessment of xylanase activity in dry storage as a potential method of reducing feedstock cost.