Document Detail


Taxol, a microtubule stabilizer, prevents atrial fibrillation in in vitro atrial fibrillation models using rabbit hearts.
MedLine Citation:
PMID:  20980952     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
BACKGROUND: Increased atrial oxidative stress plays an important role in the pathogenesis of atrial fibrillation. A predisposition for atrial fibrillation can be produced by altering the activities of enzymes related to reactive oxygen species metabolism. Microtubule integrity is necessary for cardio-protection; microtubules are involved in many cellular processes, including enzymatic activity. Taxol, a common microtubule stabilizer, can protect cardiac myocytes from oxidative stress. However, its effects on atrial fibrillation remain unknown.
MATERIAL/METHODS: In vitro rabbit heart models of ischemia-, stretch-, and cholinergic agitation-induced atrial fibrillation were developed. Using either a fluorometric or spectrophotometric assay, we measured reactive oxygen species and the activities of oxidative enzymes including nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, xanthine oxidase, mitochondrial electron transport chain complexes I, mitochondrial electron transport chain complexes III, and superoxide dismutase.
RESULTS: Taxol effectively reduced the incidence of ischemia-, stretch- and cholinergic agitation-induced atrial fibrillation in in vitro rabbit heart models by 50%, 60%, and 40%. Moreover, Taxol decreased the level of reactive oxygen species by 46%, 49%, and 70% in ischemia-, stretch- and cholinergic agitation-induced atrial fibrillation models. Additionally, the activities of NADPH oxidase and xanthine oxidase were increased, whereas those of mitochondrial electron transport chain complexes I and III and superoxide dismutase were not affected.
CONCLUSIONS: Taxol, a microtubule stabilizer, prevents atrial fibrillation in in vitro atrial fibrillation model using rabbit hearts. This stabilizer most likely prevents atrial fibrillation by reducing the level of reactive oxygen species.
Authors:
Junjie Xiao; Hong Zhang; Dandan Liang; Ying Liu; Yi Liu; Hong Zhao; Jun Li; Luying Peng; Yi-Han Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical science monitor : international medical journal of experimental and clinical research     Volume:  16     ISSN:  1643-3750     ISO Abbreviation:  Med. Sci. Monit.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9609063     Medline TA:  Med Sci Monit     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  BR353-60     Citation Subset:  IM    
Affiliation:
Department of Cardiology, East Hospital, Tongji University School of Medicine, Shanghai, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Identification of a µ opiate receptor signaling mechanism in human placenta.
Next Document:  Plasma inhibin B and antimüllerian hormone concentrations in boys: discriminating between congenita...