| Taxol alleviates 2-methoxyestradiol-induced endothelial permeability. | |
| | |
MedLine Citation:
|
PMID: 22074808 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have previously shown that the anti-cancer agent 2-methoxyestradiol (2ME) induces hyperpermeability across endothelial monolayers. Here, we show that both microtubule disruptor, 2ME, and microtubule stabilizer, paclitaxel (taxol), increase vascular lung permeability in vitro and in vivo. Simultaneous application of 2ME and taxol alleviates 2ME-induced endothelial barrier dysfunction, which is evident by the decreased Evans Blue Dye accumulation in lung tissue and increased transendothelial resistance across monolayers. 2ME significantly increases the level of p38 and MLC phosphorylation in both endothelial monolayers and murine lungs; this increase is suppressed in the presence of taxol. Taxol treatment leads to an immediate and sustained increase in tubulin acetylation in human pulmonary artery endothelial cells (HPAEC). Surprisingly, 2ME treatment also increases tubulin acetylation; however, the onset of this process is delayed and coincides with the stage of a partial barrier restoration in HPAEC monolayer. Inhibition of histone deacetylase 6 (HDAC6) with tubacin increases tubulin acetylation level, suppresses 2ME-induced HSP27 and MLC phosphorylation, and decreases 2ME-induced barrier dysfunction, suggesting barrier-protective and/or barrier-restorative role for tubulin acetylation in vascular endothelium. |
| | |
Authors:
|
Boris A Gorshkov; Marina A Zemskova; Alexander D Verin; Natalia V Bogatcheva |
Related Documents
:
|
1698638 - Stimulation of cloned human t lymphocytes via the cd3 or cd28 molecules induces enhance... 2187888 - Expression of low levels of peripheral lymph node-associated vascular addressin in muco... 7809908 - Role of endothelium in chronic inflammatory synovitis. 16831898 - Regulation of lymph node vascular growth by dendritic cells. 12499048 - Transforming growth factor-beta-inducible gene-h3 (beta(ig)-h3) promotes cell adhesion ... 2168668 - Glioma cell influence on cerebral endothelial cell na(+)-k(+)-atpase. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-10-30 |
Journal Detail:
|
Title: Vascular pharmacology Volume: 56 ISSN: 1879-3649 ISO Abbreviation: Vascul. Pharmacol. Publication Date: 2012 Jan-Feb |
Date Detail:
|
Created Date: 2012-01-30 Completed Date: 2012-08-21 Revised Date: 2013-02-20 |
Medline Journal Info:
|
Nlm Unique ID: 101130615 Medline TA: Vascul Pharmacol Country: United States |
Other Details:
|
Languages: eng Pagination: 56-63 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
|
Vascular Biology Center, Georgia Health Sciences University, Augusta, GA 30912, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylation
/
drug effects Anilides / pharmacology Animals Capillary Permeability / drug effects* Cells, Cultured Drug Interactions Endothelial Cells / drug effects*, enzymology, metabolism Endothelium, Vascular / drug effects*, enzymology, metabolism Estradiol / analogs & derivatives*, pharmacology HSP27 Heat-Shock Proteins / metabolism Histone Deacetylases / metabolism Humans Hydroxamic Acids / pharmacology Lung / drug effects, metabolism Male Mice Mice, Inbred C57BL Microtubules / metabolism Myosin Light Chains / metabolism Paclitaxel / pharmacology* Phosphorylation / drug effects Pulmonary Artery / drug effects, enzymology, metabolism Tubulin / metabolism p38 Mitogen-Activated Protein Kinases / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
R01 HL-067307/HL/NHLBI NIH HHS; R01 HL-080675/HL/NHLBI NIH HHS; R01 HL-083327/HL/NHLBI NIH HHS; R01 HL067307-08/HL/NHLBI NIH HHS; R01 HL080675-04/HL/NHLBI NIH HHS; R01 HL083327-05/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/2-methoxyoestradiol; 0/Anilides; 0/HSP27 Heat-Shock Proteins; 0/Hydroxamic Acids; 0/Myosin Light Chains; 0/Tubulin; 0/tubacin; 33069-62-4/Paclitaxel; 50-28-2/Estradiol; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases; EC 3.5.1.98/Histone Deacetylases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: No association of ALOX5AP polymorphisms with risk of MRI-defined brain infarcts.
Next Document: AMP-activated protein kinase and the control of smooth muscle cell hyperproliferation in vascular di...