| TAUTOMERIC FORMS OF METARHODOPSIN. | |
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MedLine Citation:
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PMID: 14080814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Light isomerizes the chromophore of rhodopsin, 11-cis retinal (formerly retinene), to the all-trans configuration. This introduces a succession of unstable intermediates-pre-lumirhodopsin, lumirhodopsin, metarhodopsin -in which all-trans retinal is still attached to the chromophoric site on opsin. Finally, retinal is hydrolyzed from opsin. The present experiments show that metarhodopsin exists in two tautomeric forms, metarhodopsins I and II, with lambda(max) 478 and 380 mmicro. Metarhodopsin I appears first, then enters into equilibrium with metarhodopsin II. In this equilibrium, the proportion of metarhodopsin II is favored by higher temperature or pH, neutral salts, and glycerol. The change from metarhodopsin I to II involves the binding of a proton by a group with pK 6.4 (imidazole?), and a large increase of entropy. Metarhodopsin II has been confused earlier with the final mixture of all-trans retinal and opsin (lambda(max) 387 mmicro), which it resembles in spectrum. These two products are, however, readily distinguished experimentally. |
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Authors:
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R G MATTHEWS; R HUBBARD; P K BROWN; G WALD |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of general physiology Volume: 47 ISSN: 0022-1295 ISO Abbreviation: J. Gen. Physiol. Publication Date: 1963 Nov |
Date Detail:
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Created Date: 1964-04-01 Completed Date: 1996-12-01 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 2985110R Medline TA: J Gen Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 215-40 Citation Subset: OM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Anura* Cattle* Chemical Phenomena Chemistry* Equipment and Supplies* Glycerol* Hydrogen-Ion Concentration* Light* Mollusca* Retinal Pigments* Temperature* |
| Chemical | |
Reg. No./Substance:
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0/Retinal Pigments; 56-81-5/Glycerol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Gen Physiol ISSN: 0022-1295 ISSN: 1540-7748 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright ©, 1964, by The Rockefeller Institute Press Received Day: 2 Month: 5 Year: 1963 Print publication date: Day: 1 Month: 11 Year: 1963 Volume: 47 Issue: 2 First Page: 215 Last Page: 240 ID: 2195338 PubMed Id: 14080814 |
| Tautomeric Forms of Metarhodopsin | |
| Rowena G. Matthews | |
| Ruth Hubbard | |
| Paul K. Brown | |
| George Wald | |
| From the Biological Laboratories of Harvard University, Cambridge |
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