| Taurine modulation of hypochlorous acid-induced lung epithelial cell injury in vitro. Role of anion transport. | |
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MedLine Citation:
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PMID: 8113398 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Airway secretions of cystic fibrosis patients were found to contain high concentrations of taurine, which decreased with antibiotic therapy during acute respiratory exacerbations. Taurine, in a 1:1 molar ratio with HOCl/OCl-, caused a 10-fold increase in the amount of HOCl/OCl- needed to induce cytotoxicity to the cat lung epithelial cell line, AKD. Although DMSO protected cells against HOCl/OCl(-)-mediated injury, the presence of an equimolar concentration of taurine with HOCl/OCl- prevented DMSO from protecting cells and sulfhydryl groups against oxidation, suggesting the formation of taurine chloramines. Spectral properties confirmed the formation of monochloramines and dichloramines. Chloride-free buffer, DIDS, and low temperature (4 degrees C) each protected the cells against taurine/HOCl/OCl-, indicating that taurine chloramine uptake through anion transport pathways was required to induce cytotoxicity. A molar excess of taurine inhibited cytotoxicity, to induce cytotoxicity. A molar excess of taurine inhibited cytotoxicity, by decreasing taurine dichloramines and increasing the formation of less toxic taurine monochloramines. We conclude that taurine can protect lung epithelial cells by converting HOCl/OCl- to anionic monochloramines, but that taurine dichloramines can be toxic to respiratory epithelial cells through mechanisms that depend upon epithelial cell anion transport. |
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Authors:
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A M Cantin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 93 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-03-30 Completed Date: 1994-03-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 606-14 Citation Subset: AIM; IM |
Affiliation:
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Unité de Recherche Pulmonaire, Centre Hospitalier Universitaire de Sherbrooke, Quebec, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
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pharmacology Adult Aged Animals Anions / metabolism Bronchitis / pathology Cats Cell Line Cystic Fibrosis / metabolism*, pathology Dimethyl Sulfoxide / pharmacology Epithelium / drug effects, metabolism, pathology Female Humans Hypochlorous Acid / toxicity* Lung / drug effects, metabolism, pathology* Male Reference Values Smoking / pathology Taurine / analogs & derivatives, metabolism*, pharmacology* Temperature |
| Chemical | |
Reg. No./Substance:
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0/Anions; 107-35-7/Taurine; 51036-13-6/N-chlorotaurine; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 67-68-5/Dimethyl Sulfoxide; 7790-92-3/Hypochlorous Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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