Document Detail

Taurine analog modulation of taurine uptake by two different mechanisms in cultured glial cells.
MedLine Citation:
PMID:  7857327     Owner:  NLM     Status:  MEDLINE    
Previous data have shown that HEPES, a taurine structural analog, inhibits the uptake of taurine by cultured cells differently, depending on its addition either to the culture medium or to the Krebs-Ringer buffer used for cell incubation during taurine uptake measurements (Lleu and Rebel, J Neurosci Res 23: 78-86, 1989). An extensive study of the effect of numerous other taurine structural analogs on taurine uptake by cultured glial cells was carried out. Our results show that taurine uptake modulation by structural analogs follows two different mechanisms. For the first mechanism, observable after the simultaneous presence of taurine and of its analog during the incubation time of the uptake experiment (10 min), the amine function on the molecule is essential. The sulfonate group could be replaced either by a sulfinic group or by a carboxylic group. beta-Alanine, hypotaurine, acetyltaurine, guanidinoethanesulfonate and guanidinopropionate are the most potent inhibitors in this first mechanism. For the second mechanism, which requires the presence of the analog in the culture medium during the 48 hr preceding the taurine uptake measurement, the simultaneous presence of an amine and of a sulfonate group or of an amine and a sulfinate group is required. Carboxylates are ineffective in modulating taurine uptake in this mechanism. The sulfonate buffers synthesized by Good et al. (Biochemistry 5: 467-477, 1966) also affect taurine uptake in both mechanisms.
V Petegnief; P L Lleu; R C Gupta; J J Bourguignon; G Rebel
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  49     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-03-14     Completed Date:  1995-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  399-410     Citation Subset:  IM    
Centre de Neurochimie CNRS, Strasbourg, France.
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MeSH Terms
Cells, Cultured
Culture Media
HEPES / chemistry,  pharmacology*
Neuroglia / metabolism*
Structure-Activity Relationship
Taurine / analogs & derivatives,  metabolism*
beta-Alanine / metabolism
Reg. No./Substance:
0/Culture Media; 107-35-7/Taurine; 107-95-9/beta-Alanine; 7365-45-9/HEPES

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