| Tat acetylation regulates its actions on microtubule dynamics and apoptosis in T lymphocytes. | |
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MedLine Citation:
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PMID: 20821734 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The transactivator protein Tat of human immunodeficiency virus type 1 (HIV-1) is known to suppress microtubule dynamics and thereby trigger apoptosis in T lymphocytes. These actions of Tat constitute one of the major mechanisms for the massive destruction of T lymphocytes associated with the acquired immunodeficiency syndrome. Herein, we show that Tat acetylation at lysine-28 (K28) enhances its interaction with microtubules and increases its activity to promote microtubule assembly, by lowering the critical concentration of tubulin for polymerization into microtubules. In addition, K28 acetylation enhances the ability of Tat to stabilize microtubules, leading to increased apoptosis in T lymphocytes. Our data further reveal that Tat acetylation at K28 stimulates its activity to induce the translocation of Bim, a pro-apoptotic protein of the Bcl-2 family, from microtubules to mitochondria. These findings provide the first evidence that Tat acetylation regulates its actions on microtubule dynamics and apoptosis, in addition to the regulation of its transactivation activity. |
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Authors:
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Lihong Huo; Dengwen Li; Lei Sun; Min Liu; Xingjuan Shi; Xiaoou Sun; Jingyu Li; Bin Dong; Xin Dong; Jun Zhou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-06 |
Journal Detail:
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Title: The Journal of pathology Volume: 223 ISSN: 1096-9896 ISO Abbreviation: J. Pathol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0204634 Medline TA: J Pathol Country: England |
Other Details:
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Languages: eng Pagination: 28-36 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. |
Affiliation:
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Department of Genetics and Cell Biology, Key Laboratory of Bioactive Materials of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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