Document Detail


Taste-signaling proteins are coexpressed in solitary intestinal epithelial cells.
MedLine Citation:
PMID:  17030556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The taste system, made up of taste receptor cells clustered in taste buds at the surface of the tongue and the soft palate, plays a key role in the decision to ingest or reject food and thereby is essential in protecting organisms against harmful toxins and in selecting the most appropriate nutrients. To determine if a similar chemosensory system exists in the gastrointestinal tract, we used immunohistochemistry and real-time polymerase chain reaction (PCR) to investigate which taste-signaling molecules are expressed in the intestinal mucosa. The PCR data showed that T1r1, T1r2, T1r3, alpha-gustducin, phospholipase Cbeta2 (PLCbeta2), and Trpm5 are expressed in the stomach, small intestine, and colon of mice and humans, with the exception of T1r2, which was not detected in the mouse and human stomach or in the mouse colon. Using transgenic mice expressing enhanced green fluorescent protein under the control of the Trpm5 promoter, we found colocalization of Trpm5 and alpha-gustducin in tufted cells at the surface epithelium of the colon, but these cells did not express T1r3 or PLCbeta2. In the duodenal glands, 43%, 33%, and 38% of Trpm5-expressing cells also express PLCbeta2, T1r3, or alpha-gustducin, respectively. The duodenal gland cells that coexpress PLCbeta2 and Trpm5 morphologically resemble enteroendocrine cells. We found a large degree of colocalization of Trpm5, alpha-gustducin, T1r1, and T1r3 in tufted cells of the duodenal villi, but these cells rarely expressed PLCbeta2. The data suggest that these duodenal cells are possibly involved in sensing amino acids.
Authors:
Carole Bezençon; Johannes le Coutre; Sami Damak
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Publication Detail:
Type:  Journal Article     Date:  2006-10-09
Journal Detail:
Title:  Chemical senses     Volume:  32     ISSN:  0379-864X     ISO Abbreviation:  Chem. Senses     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-20     Completed Date:  2007-02-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8217190     Medline TA:  Chem Senses     Country:  England    
Other Details:
Languages:  eng     Pagination:  41-9     Citation Subset:  IM    
Affiliation:
Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA Primers
Energy Intake
Humans
Immunohistochemistry
Intestinal Mucosa / cytology,  metabolism*
Mice
Mice, Inbred C57BL
Mice, Transgenic
Proteins / metabolism*
Signal Transduction*
Taste*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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