Document Detail


Taste bud cells of adult mice are responsive to Wnt/β-catenin signaling: implications for the renewal of mature taste cells.
MedLine Citation:
PMID:  21328519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Wnt/β-catenin signaling initiates taste papilla development in mouse embryos, however, its involvement in taste cell turnover in adult mice has not been explored. Here we used the BATGAL reporter mouse model, which carries an engineered allele in which the LacZ gene is expressed in the presence of activated β-catenin, to determine the responsiveness of adult taste bud cells to canonical Wnt signaling. Double immunostaining with markers of differentiated taste cells revealed that a subset of Type I, II, and III taste cells express β-galactosidase. Using in situ hybridization, we showed that β-catenin activates the transcription of the LacZ gene mainly in intragemmal basal cells that are immature taste cells, identified by their expression of Sonic Hedgehog (Shh). Finally, we showed that β-catenin activity is significantly reduced in taste buds of 25-week-old mice compared with 10-week-old animals. Our data suggest that Wnt/β-catenin signaling may influence taste cell turnover by regulating cell differentiation. Reduced canonical Wnt signaling in older mice could explain in part the loss of taste sensitivity with aging, implicating a possible deficiency in the rate of taste cell renewal. More investigations are now necessary to understand if and how Wnt signaling regulates adult taste cell turnover.
Authors:
Dany Gaillard; Linda A Barlow
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-04-01
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  49     ISSN:  1526-968X     ISO Abbreviation:  Genesis     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-18     Completed Date:  2011-08-09     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  295-306     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA. dany.gaillard@ucdenver.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / physiology*
Hedgehog Proteins / metabolism
Immunohistochemistry
In Situ Hybridization
Mice
Mice, Transgenic
Microscopy, Confocal
Signal Transduction / physiology*
Taste Buds / metabolism*,  physiology
Wnt Proteins / metabolism*
beta Catenin / metabolism*
beta-Galactosidase / metabolism
Grant Support
ID/Acronym/Agency:
DC008373/DC/NIDCD NIH HHS; P30 DC004657/DC/NIDCD NIH HHS; P30 DC004657/DC/NIDCD NIH HHS; R01 DC008373/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/Hedgehog Proteins; 0/Shh protein, mouse; 0/Wnt Proteins; 0/beta Catenin; EC 3.2.1.23/beta-Galactosidase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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