Document Detail


Task-oriented modular decomposition of biological networks: trigger mechanism in blood coagulation.
MedLine Citation:
PMID:  20441738     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Analysis of complex time-dependent biological networks is an important challenge in the current postgenomic era. We propose a middle-out approach for decomposition and analysis of complex time-dependent biological networks based on: 1), creation of a detailed mechanism-driven mathematical model of the network; 2), network response decomposition into several physiologically relevant subtasks; and 3), subsequent decomposition of the model, with the help of task-oriented necessity and sensitivity analysis into several modules that each control a single specific subtask, which is followed by further simplification employing temporal hierarchy reduction. The technique is tested and illustrated by studying blood coagulation. Five subtasks (threshold, triggering, control by blood flow velocity, spatial propagation, and localization), together with responsible modules, can be identified for the coagulation network. We show that the task of coagulation triggering is completely regulated by a two-step pathway containing a single positive feedback of factor V activation by thrombin. These theoretical predictions are experimentally confirmed by studies of fibrin generation in normal, factor V-, and factor VIII-deficient plasmas. The function of the factor V-dependent feedback is to minimize temporal and parametrical intervals of fibrin clot instability. We speculate that this pathway serves to lessen possibility of fibrin clot disruption by flow and subsequent thromboembolism.
Authors:
Mikhail A Panteleev; Anna N Balandina; Elena N Lipets; Mikhail V Ovanesov; Fazoil I Ataullakhanov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biophysical journal     Volume:  98     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-05     Completed Date:  2010-07-29     Revised Date:  2011-07-28    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1751-61     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Affiliation:
Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, Moscow, Russia. mapanteleev@yandex.ru
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Coagulation*
Cysteine Endopeptidases / metabolism
Factor V / metabolism
Factor VIII / metabolism
Factor X / metabolism
Feedback, Physiological
Fibrin / biosynthesis
Humans
Models, Biological*
Neoplasm Proteins / metabolism
Prothrombin / metabolism
Rabbits
Time Factors
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 9001-24-5/Factor V; 9001-26-7/Prothrombin; 9001-27-8/Factor VIII; 9001-29-0/Factor X; 9001-31-4/Fibrin; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.26/cancer procoagulant
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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