| Tartrate-resistant acid phosphatase 5a and 5b contain distinct sugar moieties. | |
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MedLine Citation:
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PMID: 18703035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tartrate-resistant acid phosphatase (TRAP) consists of 2 structurally related isoforms, 5a and 5b, and the latter has been characterized as containing a sugar moiety devoid of sialic acid(s). We provide evidence of a greater difference between the sugar moieties of the two isoforms than the presence of sialic acid(s) in TRAP 5a. TRAP 5a and 5b were highly purified from human blood and femur extracts, respectively, and after purifying the TRAPs by successive chromatography on a series of lectin affinity columns, we used the chromatographs obtained to estimate the composition of their sugar chain structure and found that both TRAP 5a and 5b had contained the heterogenous sugar chains. More specifically, TRAP 5a mainly contained a high-mannose-type sugar chain, while TRAP 5b had multi-antennary complex-type sugar chain. These results indicate that the differences between TRAP 5a and 5b consist not only of a difference in modification by sialic acid but differences in other sugar chain structures. These differences are involved in an extraordinary enzyme maturation process since the carbohydrate chains cover the repressive loop where the proteolytic site is located. |
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Authors:
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Takeshi Kawaguchi; Takanari Nakano; Kumiko Sasagawa; Tatsuya Ohashi; Toshihide Miura; Tsugikazu Komoda |
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Publication Detail:
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Type: Journal Article Date: 2008-07-30 |
Journal Detail:
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Title: Clinical biochemistry Volume: 41 ISSN: 1873-2933 ISO Abbreviation: Clin. Biochem. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-20 Completed Date: 2008-12-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0133660 Medline TA: Clin Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1245-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Faculty of Medicine, Saitama Medical University, Saitama, 350-0495, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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chemistry*,
metabolism* Carbohydrates / chemistry* Chromatography, Affinity Humans Isoenzymes / chemistry*, metabolism* Lectins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Isoenzymes; 0/Lectins; EC 3.1.3.-/tartrate-resistant acid phosphatase; EC 3.1.3.2/Acid Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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