| Targeting an ultrasound contrast agent to folate receptors on ovarian cancer cells: feasibility research for ultrasonic molecular imaging of tumor cells. | |
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MedLine Citation:
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PMID: 20375379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The purpose of the study was to synthesize and characterize folate-targeted microbubbles (MB(F)) as an ultrasound contrast agent and to evaluate their affinity to the folate receptor (FR) in vitro. METHODS: Folate-targeted microbubbles were prepared by incorporating 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]-folate into the lipid membrane of microbubbles. The diameter and concentration of the MB(F) were determined by a cell counter and sizer. The MB(F), control microbubbles (MB(C)), and MB(F) with a free folic acid block-ade were tested for binding specificity to human ovarian carcinoma SKOV3 cells, which overexpress the FR, by microscopy and confocal imaging, respectively. RESULTS: The basic physical characteristics of MB(F) were similar to those of MB(C). In the cell binding test, the adherence efficiency of MB(F) to the SKOV3 cells (mean +/- SD, 16 +/- 5 microbubbles per cell; P < .01) was significantly higher than that of MB(C) (0.7 +/- 0.4 microbubbles per cell) or MB(F) with the free folic acid blockade (0.7 +/- 0.6 microbubbles per cell). CONCLUSIONS: Folate-targeted microbubbles showed high affinity to SKOV3 cells with FR overexpression. They are potentially useful for ultrasonic molecular imaging and treatment of FR-positive tumors and warrant further investigation. |
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Authors:
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Wu Xing; Wang Zhigang; Hu Bing; Ran Haitao; Li Pan; Xu Chuanshan; Zheng Yuanyi; Li Ao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine Volume: 29 ISSN: 1550-9613 ISO Abbreviation: J Ultrasound Med Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-08 Completed Date: 2010-07-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8211547 Medline TA: J Ultrasound Med Country: United States |
Other Details:
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Languages: eng Pagination: 609-14 Citation Subset: IM |
Affiliation:
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Department of Ultrasound in Medicine, Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Contrast Media
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chemistry*,
metabolism Ethylene Glycols / chemistry*, metabolism Female Folic Acid / chemistry*, metabolism Glycerol / analogs & derivatives*, chemistry, metabolism Humans Microbubbles Microscopy, Confocal Ovarian Neoplasms / ultrasonography* Receptors, Cell Surface / chemistry*, metabolism Statistics, Nonparametric Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/1,2-distearoyl-sn-glycero-3-phosphatoethanolamine-N-(amino(poly(ethylene glycol))); 0/Contrast Media; 0/Ethylene Glycols; 0/Receptors, Cell Surface; 56-81-5/Glycerol; 59-30-3/Folic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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