Document Detail

Targeting an ultrasound contrast agent to folate receptors on ovarian cancer cells: feasibility research for ultrasonic molecular imaging of tumor cells.
MedLine Citation:
PMID:  20375379     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: The purpose of the study was to synthesize and characterize folate-targeted microbubbles (MB(F)) as an ultrasound contrast agent and to evaluate their affinity to the folate receptor (FR) in vitro. METHODS: Folate-targeted microbubbles were prepared by incorporating 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]-folate into the lipid membrane of microbubbles. The diameter and concentration of the MB(F) were determined by a cell counter and sizer. The MB(F), control microbubbles (MB(C)), and MB(F) with a free folic acid block-ade were tested for binding specificity to human ovarian carcinoma SKOV3 cells, which overexpress the FR, by microscopy and confocal imaging, respectively. RESULTS: The basic physical characteristics of MB(F) were similar to those of MB(C). In the cell binding test, the adherence efficiency of MB(F) to the SKOV3 cells (mean +/- SD, 16 +/- 5 microbubbles per cell; P < .01) was significantly higher than that of MB(C) (0.7 +/- 0.4 microbubbles per cell) or MB(F) with the free folic acid blockade (0.7 +/- 0.6 microbubbles per cell). CONCLUSIONS: Folate-targeted microbubbles showed high affinity to SKOV3 cells with FR overexpression. They are potentially useful for ultrasonic molecular imaging and treatment of FR-positive tumors and warrant further investigation.
Wu Xing; Wang Zhigang; Hu Bing; Ran Haitao; Li Pan; Xu Chuanshan; Zheng Yuanyi; Li Ao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine     Volume:  29     ISSN:  1550-9613     ISO Abbreviation:  J Ultrasound Med     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-08     Completed Date:  2010-07-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211547     Medline TA:  J Ultrasound Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  609-14     Citation Subset:  IM    
Department of Ultrasound in Medicine, Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
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MeSH Terms
Contrast Media / chemistry*,  metabolism
Ethylene Glycols / chemistry*,  metabolism
Folic Acid / chemistry*,  metabolism
Glycerol / analogs & derivatives*,  chemistry,  metabolism
Microscopy, Confocal
Ovarian Neoplasms / ultrasonography*
Receptors, Cell Surface / chemistry*,  metabolism
Statistics, Nonparametric
Tumor Cells, Cultured
Reg. No./Substance:
0/1,2-distearoyl-sn-glycero-3-phosphatoethanolamine-N-(amino(poly(ethylene glycol))); 0/Contrast Media; 0/Ethylene Glycols; 0/Receptors, Cell Surface; 56-81-5/Glycerol; 59-30-3/Folic Acid

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