Document Detail


Targeting to carcinoma cells with chitosan- and starch-coated magnetic nanoparticles for magnetic hyperthermia.
MedLine Citation:
PMID:  18257079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The delivery of hyperthermic thermoseeds to a specific target site with minimal side effects is an important challenge in targeted hyperthermia, which employs magnetic method and functional polymers. An external magnetic field is used to control the site-specific targeting of the magnetic nanoparticles. Polymer-coated magnetic nanoparticles can confer a higher affinity to the biological cell membranes. In this study, uncoated, chitosan-coated, and starch-coated magnetic nanoparticles were synthesized for use as a hyperthermic thermoseed. Each sample was examined with respect to their applications to hyperthermia using XRD, VSM, and FTIR. In addition, the temperature changes under an alternating magnetic field were observed. As in vitro tests, the magnetic responsiveness of chitosan- and starch-coated magnetite was determined by a simple blood vessel model under various intensities of magnetic field. L929 normal cells and KB carcinoma cells were used to examine the cytotoxicity and affinity of each sample using the MTT method. The chitosan-coated magnetic nanoparticles generated a higher DeltaT of 23 degrees C under an AC magnetic field than the starch-coated magnetite, and the capturing rate of the particles was 96% under an external magnetic field of 0.4 T. The highest viability of L929 cells was 93.7%. Comparing the rate of KB cells capture with the rate of L929 cells capture, the rate of KB cells capture relatively increased with 10.8% in chitosan-coated magnetic nanoparticles. Hence, chitosan-coated magnetic nanoparticles are biocompatible and have a selective affinity to KB cells. The targeting of magnetic nanoparticles in hyperthermia was improved using a controlled magnetic field and a chitosan-coating. Therefore, chitosan-coated magnetic nanoparticles are expected to be promising materials for use in magnetic targeted hyperthermia.
Authors:
Dong-Hyun Kim; Kyoung-Nam Kim; Kwang-Mahn Kim; Yong-Keun Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  88     ISSN:  1552-4965     ISO Abbreviation:  -     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-11-25     Completed Date:  2009-02-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-11     Citation Subset:  IM    
Copyright Information:
2008 Wiley Periodicals, Inc.
Affiliation:
Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 120-752, Korea.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Chitosan
Coated Materials, Biocompatible / chemistry,  therapeutic use*
Drug Delivery Systems / methods*
Fever / chemically induced*
Humans
Magnetics*
Materials Testing
Nanoparticles / administration & dosage*,  chemistry
Neoplasms / pathology
Spectroscopy, Fourier Transform Infrared
Starch
X-Ray Diffraction
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 9005-25-8/Starch; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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