Document Detail


Targeting to Fcgamma receptors, but not CR3 (CD11b/CD18), increases clearance of Bordetella pertussis.
MedLine Citation:
PMID:  11237803     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the absence of opsonizing antibodies, Bordetella pertussis, the causative agent of pertussis, readily binds to phagocytes via complement receptor 3 (CR3). After opsonization with antibodies, binding is mediated by IgG receptors (FcgammaR). The effect of targeting B. pertussis to either FcgammaR or CR3 was studied. The fate of unopsonized B. pertussis, IgG-opsonized B. pertussis, and B. pertussis opsonized with bispecific antibodies (BsAbs) directed to CR3 or FcgammaRII/-III was compared. IgG antibodies mediated binding and phagocytosis of B. pertussis via FcgammaR by polymorphonuclear leukocytes (PMNL) in vitro. Opsonization of B. pertussis with BsAbs directed against either CR3 or FcgammaRII/-III facilitated PMNL phagocytosis; however, in vivo studies with BsAb revealed that FcgammaR-mediated uptake facilitates B. pertussis clearance, in contrast to uptake via CR3. Targeting of B. pertussis to FcgammaRII/-III in mice deficient in FcgammaRII or FcgammaRIII indicated that the protective effect is attributable to FcgammaRIII. Competition between uptake via CR3 or FcgammaR may determine the outcome of natural infection.
Authors:
S M Hellwig; H F van Oirschot; W L Hazenbos; A B van Spriel; F R Mooi; J G van De Winkel
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2001-02-13
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  183     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-12     Completed Date:  2001-04-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  871-9     Citation Subset:  AIM; IM    
Affiliation:
Laboratory for Infectious Diseases. National Institute of Public Health and the Environment, Bilthoven, Utrecht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Bacterial / immunology*
Antibodies, Bispecific / immunology
Bordetella pertussis / immunology*
Cells, Cultured
Female
Immunoglobulin G / immunology
Macrophage-1 Antigen / genetics,  immunology*
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Neutrophils / immunology
Opsonin Proteins / immunology
Phagocytosis*
Receptors, IgG / genetics,  immunology*
Whooping Cough / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antibodies, Bispecific; 0/Immunoglobulin G; 0/Macrophage-1 Antigen; 0/Opsonin Proteins; 0/Receptors, IgG

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