Document Detail


Targeting and penetration of virus receptor bearing cells by nanoparticles coated with envelope proteins of Moloney murine leukemia virus.
MedLine Citation:
PMID:  17090066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One of the most important steps in a productive viral infection is when the virus fuses to a cell membrane and delivers its genome into the cell cytosol. This dynamic event is mediated by interactions between specific virus envelope proteins with their cell-bound receptors. This process is exemplified by Moloney murine leukemia virus (Mo-MLV) where envelope protein interaction with its receptor, mCAT-1, leads to virus-cell membrane fusion and infection of cells. Here, fluorescent nanoparticles (NPs) were coated with Mo-MLV derived membranes (Mo-NPs) by extrusion. Electron microscopy and biochemical analysis showed tight association of the virus membranes and NPs. The coated NPs mimic native virus by binding and entering only cells expressing the virus receptor. Confocal microscopy revealed that the coated NPs were taken up into endocytic compartments containing receptor and were also seen associated with caveolin, a marker of caveolae. To demonstrate that the Mo-NPs could escape endosomes and deliver a protein cargo into the cell cytosol, beta-lactamase (betalac) was covalently coupled to the Mo-NP cores and incubated with cells. betalac activity was only detected in the cytosol of mCAT-1-expressing cells. This is the first time that virus proteins have been used to specifically target NPs to receptor-bearing cells as well as penetration into the cell cytosol. Extrusion provides a rapid, detergent-free method to couple virus membranes to NPs and should be readily applicable for many other virus and NP types.
Authors:
Drew C Deniger; Andrey A Kolokoltsov; Andrew C Moore; Thomas B Albrecht; Robert A Davey
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Nano letters     Volume:  6     ISSN:  1530-6984     ISO Abbreviation:  Nano Lett.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-08     Completed Date:  2007-01-23     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  101088070     Medline TA:  Nano Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2414-21     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cationic Amino Acid Transporter 1 / chemistry*,  metabolism
Cell Line
Cell Membrane / chemistry,  metabolism
Cytosol / chemistry,  metabolism
Humans
Mice
Moloney murine leukemia virus / chemistry*,  metabolism
Nanoparticles / chemistry*
Particle Size
Surface Properties
Grant Support
ID/Acronym/Agency:
R01 AI063513/AI/NIAID NIH HHS; U54 AI057156/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Cationic Amino Acid Transporter 1

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