| Targeting and penetration of virus receptor bearing cells by nanoparticles coated with envelope proteins of Moloney murine leukemia virus. | |
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MedLine Citation:
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PMID: 17090066 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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One of the most important steps in a productive viral infection is when the virus fuses to a cell membrane and delivers its genome into the cell cytosol. This dynamic event is mediated by interactions between specific virus envelope proteins with their cell-bound receptors. This process is exemplified by Moloney murine leukemia virus (Mo-MLV) where envelope protein interaction with its receptor, mCAT-1, leads to virus-cell membrane fusion and infection of cells. Here, fluorescent nanoparticles (NPs) were coated with Mo-MLV derived membranes (Mo-NPs) by extrusion. Electron microscopy and biochemical analysis showed tight association of the virus membranes and NPs. The coated NPs mimic native virus by binding and entering only cells expressing the virus receptor. Confocal microscopy revealed that the coated NPs were taken up into endocytic compartments containing receptor and were also seen associated with caveolin, a marker of caveolae. To demonstrate that the Mo-NPs could escape endosomes and deliver a protein cargo into the cell cytosol, beta-lactamase (betalac) was covalently coupled to the Mo-NP cores and incubated with cells. betalac activity was only detected in the cytosol of mCAT-1-expressing cells. This is the first time that virus proteins have been used to specifically target NPs to receptor-bearing cells as well as penetration into the cell cytosol. Extrusion provides a rapid, detergent-free method to couple virus membranes to NPs and should be readily applicable for many other virus and NP types. |
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Authors:
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Drew C Deniger; Andrey A Kolokoltsov; Andrew C Moore; Thomas B Albrecht; Robert A Davey |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Nano letters Volume: 6 ISSN: 1530-6984 ISO Abbreviation: Nano Lett. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-08 Completed Date: 2007-01-23 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 101088070 Medline TA: Nano Lett Country: United States |
Other Details:
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Languages: eng Pagination: 2414-21 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cationic Amino Acid Transporter 1 / chemistry*, metabolism Cell Line Cell Membrane / chemistry, metabolism Cytosol / chemistry, metabolism Humans Mice Moloney murine leukemia virus / chemistry*, metabolism Nanoparticles / chemistry* Particle Size Surface Properties |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI063513/AI/NIAID NIH HHS; U54 AI057156/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cationic Amino Acid Transporter 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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