Document Detail


Targeting multi-stranded DNA structures.
MedLine Citation:
PMID:  10637359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The design of agents targeted toward a structure-specific molecular recognition of DNA triplexes or tetraplexes ( quadruplexes ) is discussed, where such structures are relevant to antigene-based chemotherapies and the in situ cellular inhibition of telomerase function, respectively. Using principles that stem from the development of earlier synthetic duplex-binding ligands, together with recent findings that probe structure thermodynamic linkages and kinetic features of stability, a rational approach is developed to exploit the distinct molecular templates offered by these high-order nucleic acid biotarget systems. Such analytical techniques can usefully augment conventional drug design methods, particularly where detailed structural information is unavailable or the mode of binding to form a persistent DNA biotarget ligand complex is not established. Examples from the author s laboratory are used to illustrate structure-specific (or structure-preferential) recognition and subsequent stabilization of DNA triplexes using intercalative or groove-mediated binding mechanisms, and the successful targeting of DNA tetraplexes using planar extended-aromatic ligands. In each case, chemical manipulation of the molecule by exploiting either (i) geometric isomers, (ii) redistribution of charged groups and/or H-bond donors/acceptors, or (iii) optimization of intermolecular pi-overlap can be used to improve the affinity or specificity of the underlying DNA drug binding events.
Authors:
T C Jenkins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Current medicinal chemistry     Volume:  7     ISSN:  0929-8673     ISO Abbreviation:  Curr. Med. Chem.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-17     Completed Date:  2000-02-17     Revised Date:  2007-02-12    
Medline Journal Info:
Nlm Unique ID:  9440157     Medline TA:  Curr Med Chem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  99-115     Citation Subset:  IM    
Affiliation:
Yorkshire Cancer Research Laboratory of Drug Design, University Cancer Medicine Unit, University of Bradford, Bradford, West Yorkshire, BD7 1DP, UK. t.c.jenkins@brad.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA / chemistry,  drug effects*,  metabolism
Humans
Intercalating Agents / pharmacology
Ligands
Chemical
Reg. No./Substance:
0/Intercalating Agents; 0/Ligands; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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