| Targeting of the mouse Slc39a2 (Zip2) gene reveals highly cell-specific patterns of expression, and unique functions in zinc, iron, and calcium homeostasis. | |
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MedLine Citation:
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PMID: 17506078 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fourteen members of the Slc39a superfamily of metal ion uptake transporters have been identified in mice and humans, but the physiological functions of most remain obscure. Herein, we created mice with Zip2 (Slc39a2) genes in which the open reading frame was replaced with that of the enhanced green fluorescent protein (EGFP), to study temporal and spatial patterns of Zip2 gene expression and examine the physiological roles of this transporter. Expression of this gene was remarkably cell-type specific and developmentally regulated in pericentral hepatocytes, developing keratinocytes, and a subset of immature dendritic cells in the immune system. In addition, the Zip2 gene was transiently expressed in giant trophoblast cells in the placenta. Although the Zip2 gene was not essential under conditions of normal dietary zinc, it played an important role in adapting to dietary zinc deficiency during pregnancy, and in the homeostasis of iron in the liver as well as iron and calcium in developing embryos. These studies suggest that active expression of the Zip2 gene in these few specific cell types, aforementioned, plays a particularly important role during zinc deficiency. These studies further reveal novel interactions between zinc transporter function and the homeostasis of other essential metals. |
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Authors:
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Jennifer L Peters; Jodi Dufner-Beattie; Wenhao Xu; Jim Geiser; Brett Lahner; David E Salt; Glen K Andrews |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Genesis (New York, N.Y. : 2000) Volume: 45 ISSN: 1526-954X ISO Abbreviation: Genesis Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-12 Completed Date: 2007-08-29 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 100931242 Medline TA: Genesis Country: United States |
Other Details:
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Languages: eng Pagination: 339-52 Citation Subset: IM |
Copyright Information:
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Published 2007 Wiley-Liss, Inc. |
Affiliation:
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Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160-7421, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism Cation Transport Proteins / genetics, physiology* Dendritic Cells / chemistry, metabolism Female Gene Expression Gene Expression Regulation, Developmental* Gene Targeting Green Fluorescent Proteins / analysis, genetics Hepatocytes / chemistry, metabolism Homeostasis Iron / metabolism Keratinocytes / chemistry, metabolism Mice Mice, Knockout Pregnancy Trophoblasts / chemistry, metabolism Zinc / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DK59369/DK/NIDDK NIH HHS; P20RR016443/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cation Transport Proteins; 0/Slc39a2 protein, mouse; 147336-22-9/Green Fluorescent Proteins; 7439-89-6/Iron; 7440-66-6/Zinc; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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