Document Detail

Targeting gemcitabine containing liposomes to CD44 expressing pancreatic adenocarcinoma cells causes an increase in the antitumoral activity.
MedLine Citation:
PMID:  23384419     Owner:  NLM     Status:  Publisher    
Pancreatic adenocarcinoma is often diagnosed when metastatic events have occurred. The early spread of circulating cancer cells expressing the CD44 receptor may play a crucial role in this process. In this study, we have investigated the cellular delivery ability and both in vitro and in vivo anti-tumoural activity of liposomes conjugated with two different low molecular weight hyaluronic acid (HA 4.8kDa and HA 12kDa), the primary ligand of CD44, and containing a lipophilic gemcitabine (GEM) pro-drug. By confocal microscopy and flow cytometry analyses, we demonstrate that the cellular uptake into a highly CD44-expressing pancreatic adenocarcinoma cell line is higher with HA-conjugated (12kDa>4.8kDa) than non-conjugated liposomes. Consistently, in vitro cytotoxic assays display an increased sensitivity towards GEM containing HA-liposomes, compared to non-conjugated liposomes. Conversely, CD44 non-expressing normal cells show a similar uptake and in vitro cytotoxicity with both HA-conjugated and non-conjugated liposomes. Furthermore, we demonstrate that the HA-liposomes are taken up into the cells via lipid raft-mediated endocytosis. All the liposome formulations containing GEM show a higher antitumoral activity than free GEM in a mouse xenograft tumour model of human pancreatic adenocarcinoma. The 12kDa HA-liposomes have the strongest efficiency, while non-conjugated liposomes and the 4.8kDa HA-liposomes are similarly active. Taken together, our results provide a strong rationale for further development of HA-conjugated liposomes to treat pancreatic adenocarcinoma.
Elisa Dalla Pozza; Carlotta Lerda; Chiara Costanzo; Massimo Donadelli; Ilaria Dando; Elisa Zoratti; Maria Teresa Scupoli; Stefania Beghelli; Aldo Scarpa; Elias Fattal; Silvia Arpicco; Marta Palmieri
Related Documents :
17947299 - Sodium-potassium atpase 1 subunit is a molecular partner of wolframin, an endoplasmic r...
8254819 - Influence of growth factors on in vitro invasiveness and type iv collagenolysis of huma...
2112899 - Measurement of beta-galactosyltransferase activity in cell extracts with an elisa-based...
16505219 - The mesenchyme controls the timing of pancreatic beta-cell differentiation.
20087799 - Aminochrome induces disruption of actin, alpha-, and beta-tubulin cytoskeleton networks...
12079519 - Immunocytochemical expression of microtubule-associated protein-2 (map-2) in small cell...
12490299 - Embryonic liver cells and permanent lines as models for hepatocyte and bile duct cell d...
17947299 - Sodium-potassium atpase 1 subunit is a molecular partner of wolframin, an endoplasmic r...
11991179 - Production of core and virus-like particles with baculovirus infected insect cells.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-2
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  -     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Department of Life and Reproduction Sciences, Biochemistry Section, University of Verona, Verona, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The electrical response of bilayers to the bee venom toxin melittin: evidence for transient bilayer ...
Next Document:  Doctors are to blame for perceived medical adverse events. A cross sectional population study. The T...