Document Detail


Targeting bacterial integration host factor to disrupt biofilms associated with cystic fibrosis.
MedLine Citation:
PMID:  23168017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: This study aims to identify whether the bacterial protein, Integration Host Factor (IHF), is present within sputum solids collected from cystic fibrosis (CF) patients and thus might contribute to the structural stability of biofilms within the lungs.
METHODS: The presence of IHF in sputum was determined by immunohistochemistry. The role of IHF in stabilizing biofilms within sputum was tested in vitro wherein anti-IHF was used to attempt to dissolve sputum solids.
RESULTS: Thirty-seven of 44 sputum samples (84%) were positive for anti-IHF staining. Treatment with anti-IHF or DNase of 6 representative samples, dissolved sputum solids significantly better than treatment with normal saline in vitro, and strong synergism was observed when these agents were used in combination.
CONCLUSIONS: IHF was detected in the majority of sputum samples from patients with CF and in vitro treatment with anti-IHF induced dissolution of sputum solids. These data support further investigation of IHF as a potential therapeutic target for patients with CF.
Authors:
Jodi E Gustave; Joseph A Jurcisek; Karen S McCoy; Steven D Goodman; Lauren O Bakaletz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-11-17
Journal Detail:
Title:  Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society     Volume:  12     ISSN:  1873-5010     ISO Abbreviation:  J. Cyst. Fibros.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-05-31     Completed Date:  2013-10-28     Revised Date:  2014-07-02    
Medline Journal Info:
Nlm Unique ID:  101128966     Medline TA:  J Cyst Fibros     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  384-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Biofilms*
Child
Child, Preschool
Cystic Fibrosis* / drug therapy
Female
Humans
Integration Host Factors / analysis*,  antagonists & inhibitors
Male
Middle Aged
Sputum / chemistry*
Young Adult
Grant Support
ID/Acronym/Agency:
R01 DC 011818/DC/NIDCD NIH HHS; R01 DC011818/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/Integration Host Factors
Comments/Corrections

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