Document Detail

Targeting antigen to bone marrow stromal cell-2 expressed by conventional and plasmacytoid dendritic cells elicits efficient antigen presentation.
MedLine Citation:
PMID:  23303646     Owner:  NLM     Status:  MEDLINE    
Bone marrow stromal cell-2 (BST-2) has major roles in viral tethering and modulation of interferon production. Here we investigate BST-2 as a receptor for the delivery of antigen to dendritic cells (DCs). We show that BST-2 is expressed by a panel of mouse and human DC subsets, particularly under inflammatory conditions. The outcome of delivering antigen to BST-2 expressed by steady state and activated plasmacytoid DC (pDC) or conventional CD8(+) and CD8(-) DCs was determined. T-cell responses were measured for both MHC class I (MHCI) and MHC class II (MHCII) antigen presentation pathways in vitro. Delivering antigen via BST-2 was compared with that via receptors DEC205 or Siglec-H. We show that despite a higher antigen load and faster receptor internalisation, when antigen is delivered to steady state or activated pDC via BST-2, BST-2-targeted activated conventional DCs present antigen more efficiently. Relative to DEC205, BST-2 was inferior in its capacity to deliver antigen to the MHCI cross-presentation pathway. In contrast, BST-2 was superior to Siglec-H at initiating either MHCI or MHCII antigen presentation. In summary, BST-2 is a useful receptor to target with antigen, given its broad expression pattern and ability to access both MHCI and MHCII presentation pathways with relative efficiency.
Jessica M Moffat; Elodie Segura; Gabriela Khoury; Irene Caminschi; Paul U Cameron; Sharon R Lewin; Jose A Villadangos; Justine D Mintern
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-02-06
Journal Detail:
Title:  European journal of immunology     Volume:  43     ISSN:  1521-4141     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-13     Completed Date:  2013-05-02     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  595-605     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Antigen Presentation / immunology*
Antigens / immunology,  metabolism
Antigens, CD / genetics*,  immunology,  metabolism*
Antigens, Surface / genetics,  metabolism
Dendritic Cells / immunology*,  metabolism*
GPI-Linked Proteins / genetics,  immunology,  metabolism
Gene Expression Profiling
Membrane Glycoproteins / genetics*,  immunology,  metabolism*
Receptors, Cell Surface / metabolism
Transcription, Genetic
Reg. No./Substance:
0/Antigens; 0/Antigens, CD; 0/Antigens, Surface; 0/BST2 protein, human; 0/BST2 protein, mouse; 0/GPI-Linked Proteins; 0/Membrane Glycoproteins; 0/Receptors, Cell Surface

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