Document Detail


Targeting the Wnt signaling pathway with dishevelled and cisplatin synergistically suppresses mesothelioma cell growth.
MedLine Citation:
PMID:  18225596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: We have previously found that Wnt signaling is activated in mesothelioma cells. To clarify the effect of blocking Wnt signaling in mesothelioma, the expression of dishevelled (Dvl), an intermediator of Wnt signaling, was down-regulated by a reformed type of small interfering RNA (siRNA), stealth RNAi, which can reduce the cytotoxic interferon response unlike conventional siRNA. MATERIALS AND METHODS: Mesothelioma cell lines were transfected with stealth RNAi of Dvl, and cell growth and colony formation were examined. The synergistic effect on cell growth of Dvl stealth RNAi and cisplatin in combination was evaluated. RESULTS: Dvl stealth RNAi down-regulated the expression of Dvl-3 in mesothelioma cells and induced cell cycle aberration which caused suppression of cell growth. Colony formation was also suppressed. Dvl stealth RNAi and cisplatin in combination suppressed cell growth synergistically. CONCLUSION: Our data suggest that inhibition of Wnt signaling leads to significant antitumor effects.
Authors:
Kazutsugu Uematsu; Nobuhiko Seki; Takashi Seto; Chie Isoe; Hideo Tsukamoto; Iwao Mikami; Liang You; Biao He; Zhidong Xu; David M Jablons; Kenji Eguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  27     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2008-01-29     Completed Date:  2008-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  4239-42     Citation Subset:  IM    
Affiliation:
Division of Clinical Oncology, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan. kuematsu@saitama-med.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / biosynthesis,  genetics*,  metabolism
Cell Growth Processes
Cisplatin / pharmacology*
Combined Modality Therapy
Down-Regulation
Humans
Mesothelioma / drug therapy*,  genetics*,  metabolism,  pathology
Phosphoproteins / biosynthesis,  genetics*,  metabolism
RNA, Small Interfering / genetics
Signal Transduction
Wnt Proteins / metabolism*
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Phosphoproteins; 0/RNA, Small Interfering; 0/Wnt Proteins; 0/dishevelled proteins; 15663-27-1/Cisplatin

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