Document Detail


Targeted zwitterionic near-infrared fluorophores for improved optical imaging.
MedLine Citation:
PMID:  23292608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The signal-to-background ratio (SBR) is the key determinant of sensitivity, detectability and linearity in optical imaging. As signal strength is often constrained by fundamental limits, background reduction becomes an important approach for improving the SBR. We recently reported that a zwitterionic near-infrared (NIR) fluorophore, ZW800-1, exhibits low background. Here we show that this fluorophore provides a much-improved SBR when targeted to cancer cells or proteins by conjugation with a cyclic RGD peptide, fibrinogen or antibodies. ZW800-1 outperforms the commercially available NIR fluorophores IRDye800-CW and Cy5.5 in vitro for immunocytometry, histopathology and immunoblotting and in vivo for image-guided surgery. In tumor model systems, a tumor-to-background ratio of 17.2 is achieved at 4 h after injection of ZW800-1 conjugated to cRGD compared to ratios of 5.1 with IRDye800-CW and 2.7 with Cy5.5. Our results suggest that introducing zwitterionic properties into targeted fluorophores may be a general strategy for improving the SBR in diagnostic and therapeutic applications.
Authors:
Hak Soo Choi; Summer L Gibbs; Jeong Heon Lee; Soon Hee Kim; Yoshitomo Ashitate; Fangbing Liu; Hoon Hyun; GwangLi Park; Yang Xie; Soochan Bae; Maged Henary; John V Frangioni
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-06
Journal Detail:
Title:  Nature biotechnology     Volume:  31     ISSN:  1546-1696     ISO Abbreviation:  Nat. Biotechnol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-08     Completed Date:  2013-09-04     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  9604648     Medline TA:  Nat Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  148-53     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / isolation & purification
Cell Line, Tumor
Fibrinogen / isolation & purification
Fluorescent Dyes / chemistry
Humans
Mice
Mice, Nude
Microscopy, Fluorescence
Neoplasms / diagnosis,  pathology*
Optical Imaging / methods*
Peptides, Cyclic / isolation & purification
Quaternary Ammonium Compounds / chemistry,  diagnostic use*
Signal-To-Noise Ratio
Sulfonic Acids / chemistry,  diagnostic use*
Surgery, Computer-Assisted
Grant Support
ID/Acronym/Agency:
R01 CA115296/CA/NCI NIH HHS; R01 EB010022/EB/NIBIB NIH HHS; R01 EB011523/EB/NIBIB NIH HHS; R01-CA-115296/CA/NCI NIH HHS; R01-EB-010022/EB/NIBIB NIH HHS; R01-EB-011523/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Fluorescent Dyes; 0/Peptides, Cyclic; 0/Quaternary Ammonium Compounds; 0/Sulfonic Acids; 0/ZW800-1 compound; 0/cyclic arginine-glycine-aspartic acid peptide; 9001-32-5/Fibrinogen
Comments/Corrections
Comment In:
Nat Methods. 2013 Mar;10(3):196   [PMID:  23570044 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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