Document Detail

Targeted sequence alteration of a chromosomal locus in mouse liver.
MedLine Citation:
PMID:  20025952     Owner:  NLM     Status:  MEDLINE    
Targeted sequence alteration would be an attractive method in gene therapy and biotechnology. To achieve in vivo targeted sequence alteration, a tailed duplex DNA consisting of annealed 35mer and 794mer single-stranded DNAs was delivered by means of hydrodynamic tail vein injection into liver of transgenic mouse harboring a reporter gene (the rpsL gene) in its genome. The tailed DNA was designed for a conversion of ATC to AGC at codon 80 of the rpsL transgene. The anticipated T-->G sequence alteration was induced in the transgene in the liver with an efficiency of approximately 0.1%. These results demonstrate the significant potential of this method for applications in gene therapy and biotechnology.
Hiroyuki Kamiya; Masayuki Uchiyama; Jingshu Piao; Yoshimichi Nakatsu; Teruhisa Tsuzuki; Hideyoshi Harashima
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-16
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  387     ISSN:  1873-3476     ISO Abbreviation:  Int J Pharm     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-15     Completed Date:  2010-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  180-3     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Base Sequence
Biotechnology / methods
Chromosomes, Mammalian / genetics*
DNA, Single-Stranded / administration & dosage*
Gene Therapy / methods
Genes, Reporter
Liver / metabolism*
Mice, Transgenic
Sequence Analysis, DNA
Reg. No./Substance:
0/DNA, Single-Stranded

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effect of 1,4-cyclohexanediol on percutaneous absorption and penetration of azelaic acid.
Next Document:  Gastroprotective effect of barbatusin and 3-beta-hydroxy-3-deoxibarbatusin, quinonoid diterpenes iso...