Document Detail


Targeted sequence alteration of a chromosomal locus in mouse liver.
MedLine Citation:
PMID:  20025952     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Targeted sequence alteration would be an attractive method in gene therapy and biotechnology. To achieve in vivo targeted sequence alteration, a tailed duplex DNA consisting of annealed 35mer and 794mer single-stranded DNAs was delivered by means of hydrodynamic tail vein injection into liver of transgenic mouse harboring a reporter gene (the rpsL gene) in its genome. The tailed DNA was designed for a conversion of ATC to AGC at codon 80 of the rpsL transgene. The anticipated T-->G sequence alteration was induced in the transgene in the liver with an efficiency of approximately 0.1%. These results demonstrate the significant potential of this method for applications in gene therapy and biotechnology.
Authors:
Hiroyuki Kamiya; Masayuki Uchiyama; Jingshu Piao; Yoshimichi Nakatsu; Teruhisa Tsuzuki; Hideyoshi Harashima
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-16
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  387     ISSN:  1873-3476     ISO Abbreviation:  Int J Pharm     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-15     Completed Date:  2010-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  180-3     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Affiliation:
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan. hirokam@pharm.hokudai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Biotechnology / methods
Chromosomes, Mammalian / genetics*
DNA, Single-Stranded / administration & dosage*
Female
Gene Therapy / methods
Genes, Reporter
Genome
Liver / metabolism*
Male
Mice
Mice, Transgenic
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/DNA, Single-Stranded

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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