Document Detail


Targeted nanoparticles enhanced flow electroporation of antisense oligonucleotides in leukemia cells.
MedLine Citation:
PMID:  20630739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Liposome nanoparticles (LNs) with a targeting ligand were used in a semi-continuous flow electroporation (SFE) device to enhance in vitro delivery of exogenous oligonucleotides (ODN). Nanoparticles comprising transferrin-targeted lipoplex encapsulating ODN G3139 were mixed with K562 cells (a chronic myeloid leukemia cell line) and incubated for half an hour to accomplish nanoparticle binding. The mixture was then flowed through a SFE channel where electric pulses were given. Better ODN delivery efficiency was achieved with an increase of ∼24% to the case in combination of non-targeted LNs and SFE, and ∼60% to the case using targeted LNs alone, respectively. The MTS assay results confirmed cell viability greater than 75%.
Authors:
Shengnian Wang; Xulang Zhang; Bo Yu; Robert J Lee; L James Lee
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-07-01
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  26     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-02-07     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  778-83     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Chemical Engineering Program, Louisiana Tech University, Ruston, Louisiana 71272, United States.
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MeSH Terms
Descriptor/Qualifier:
DNA, Antisense / administration & dosage,  genetics*,  pharmacokinetics*
Electroporation / instrumentation*
Equipment Design
Equipment Failure Analysis
Flow Injection Analysis / instrumentation*
Gene Targeting / instrumentation*
Humans
K562 Cells
Transfection / instrumentation*
Grant Support
ID/Acronym/Agency:
R21 EB008247/EB/NIBIB NIH HHS; R21EB008247/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Antisense
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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