| Targeted mutagenesis of the endogenous mouse Mis gene promoter: in vivo definition of genetic pathways of vertebrate sexual development. | |
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MedLine Citation:
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PMID: 10571183 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mutations were introduced into conserved steroidogenic factor 1 (SF1)- and SOX9-binding sites within the endogenous mouse Mullerian inhibiting substance (Mis) promoter. Male mice homozygous for the mutant SF1-binding site correctly initiated Mis transcription in fetal testes, although at significantly reduced levels. Surprisingly, sufficient MIS was produced to eliminate the MUllerian ducts. In contrast, males homozygous for the mutant SOX9-binding site did not initiate Mis transcription, resulting in pseudohermaphrodites. These studies suggest an essential role for SOX9 in the initiation of Mis transcription, whereas SF1 appears to act as a quantitative regulator of Mis transcript levels, perhaps for influencing non-Mullerian duct tissues. Comparative studies of Mis expression in vertebrates indicate that the Mis promoter receives transcriptional inputs that vary between species but result in the same functional readout. |
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Authors:
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N A Arango; R Lovell-Badge; R R Behringer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell Volume: 99 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 1999 Nov |
Date Detail:
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Created Date: 1999-12-14 Completed Date: 1999-12-14 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 409-19 Citation Subset: IM |
Affiliation:
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Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Mullerian Hormone Binding Sites DNA-Binding Proteins / metabolism* Female Fushi Tarazu Transcription Factors Gene Targeting Glycoproteins* Growth Inhibitors / genetics*, physiology High Mobility Group Proteins / metabolism* Homeodomain Proteins Male Mice Mice, Inbred C57BL Mice, Transgenic Mullerian Ducts / physiology Mutagenesis Promoter Regions, Genetic* Receptors, Cytoplasmic and Nuclear SOX9 Transcription Factor Sexual Maturation / physiology* Steroidogenic Factor 1 Testicular Hormones / genetics*, physiology Transcription Factors / metabolism* Transcription, Genetic Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
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CA09299/CA/NCI NIH HHS; HD30284/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Fushi Tarazu Transcription Factors; 0/Glycoproteins; 0/Growth Inhibitors; 0/High Mobility Group Proteins; 0/Homeodomain Proteins; 0/Receptors, Cytoplasmic and Nuclear; 0/SOX9 Transcription Factor; 0/Sox9 protein, mouse; 0/Steroidogenic Factor 1; 0/Testicular Hormones; 0/Transcription Factors; 0/steroidogenic factor 1, mouse; 80497-65-0/Anti-Mullerian Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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