| Targeted mutagenesis of acyl-lipid desaturases in Synechocystis: evidence for the important roles of polyunsaturated membrane lipids in growth, respiration and photosynthesis. | |
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MedLine Citation:
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PMID: 8978669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Acyl-lipid desaturases introduce double bonds (unsaturated bonds) at specifically defined positions in fatty acids that are esterified to the glycerol backbone of membrane glycerolipids. The desA, desB and desD genes of Synechocystis sp. PCC 6803 encode acyl-lipid desaturases that introduce double bonds at the delta12, omega3 and delta6 positions of C18 fatty acids respectively. The mutation of each of these genes by insertion of an antibiotic resistance gene cartridge completely eliminated the corresponding desaturation reaction. This system allowed us to manipulate the number of unsaturated bonds in membrane glycerolipids in this organism in a step-wise manner. Comparisons of the variously mutated cells revealed that the replacement of all polyunsaturated fatty acids by a monounsaturated fatty acid suppressed growth of the cells at low temperature and, moreover, it decreased the tolerance of the cells to photoinhibition of photosynthesis at low temperature by suppressing recovery of the photosystem II protein complex from photoinhibitory damage. However, the replacement of tri- and tetraunsaturated fatty acids by a diunsaturated fatty acid did not have such effects. These findings indicate that polyunsaturated fatty acids are important in protecting the photosynthetic machinery from photoinhibition at low temperatures. |
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Authors:
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Y Tasaka; Z Gombos; Y Nishiyama; P Mohanty; T Ohba; K Ohki; N Murata |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The EMBO journal Volume: 15 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-01-27 Completed Date: 1997-01-27 Revised Date: 2010-09-13 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 6416-25 Citation Subset: IM |
Affiliation:
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Department of Regulation Biology, National Institute for Basic Biology, Myodaiji, Okazaki, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calorimetry, Differential Scanning Chlorophyll / metabolism Cyanobacteria / enzymology*, genetics, growth & development Fatty Acid Desaturases / genetics, metabolism* Fatty Acids, Unsaturated / metabolism* Genes, Bacterial Glycerides / metabolism Kinetics Membrane Lipids / metabolism* Mutagenesis, Insertional Oxygen Consumption* Photosynthesis* Plasmids Recombinant Proteins / metabolism Temperature |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Glycerides; 0/Membrane Lipids; 0/Recombinant Proteins; 1406-65-1/Chlorophyll; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-9 fatty acid desaturase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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