Document Detail


Targeted modulation of sinapine biosynthesis pathway for seed quality improvement in Brassica napus.
MedLine Citation:
PMID:  18612839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arabidopsis thaliana and other members of the Brassicaceae accumulate the hydroxycinnamic acid esters sinapoylmalate in leaves and sinapoylcholine in seeds. Our recent understanding of the phenylpropanoid pathway although complex has enabled us to perturb the sinapine biosynthesis pathway in plants. Sinapine (sinapoylcholine) is the most abundant antinutritional phenolic compound in seeds of cruciferous species and therefore is a target for elimination in canola (Brassica napus) meal. We analysed A. thaliana mutants with specific blocks in the phenylpropanoid pathway and identified mutant lines with significantly altered sinapine content. Knowledge gained from A. thaliana was extended to B. napus and the corresponding phenylpropanoid pathway genes were manipulated to disrupt sinapine biosynthesis in B. napus. Based on our understanding of the A. thaliana genetics, we have successfully developed transgenic B. napus lines with ferulic acid 5-hydroxylase (FAH) and sinapoylglucose:choline sinapoyltransferase (SCT)-antisense. These lines with concomitant downregulation of FAH and SCT showed up to 90% reduction in sinapine. In addition to reduced sinapine content, we detected higher levels of free choline accumulation in the seeds. These results indicate that it is possible to develop plants with low sinapine and higher choline by manipulating specific steps in the biosynthetic pathway. These improvements are important to add value to canola meal for livestock feed.
Authors:
V-S Bhinu; Ulrike A Schäfer; Rong Li; Jun Huang; Abdelali Hannoufa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-09
Journal Detail:
Title:  Transgenic research     Volume:  18     ISSN:  0962-8819     ISO Abbreviation:  Transgenic Res.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-15     Completed Date:  2009-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9209120     Medline TA:  Transgenic Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  31-44     Citation Subset:  IM    
Affiliation:
Genomics and Biotechnology, Agriculture and Agri-Food Canada.
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MeSH Terms
Descriptor/Qualifier:
Acyltransferases / physiology*
Biosynthetic Pathways*
Brassica napus / genetics*,  growth & development,  metabolism
Choline / analogs & derivatives*,  metabolism
Coumaric Acids / metabolism
Mixed Function Oxygenases / physiology*
RNA, Small Interfering / pharmacology
Seeds / chemistry*
Transformation, Genetic
Chemical
Reg. No./Substance:
0/Coumaric Acids; 0/RNA, Small Interfering; 1135-24-6/ferulic acid; 18696-26-9/sinapine; 62-49-7/Choline; EC 1.-/Mixed Function Oxygenases; EC 2.3.-/Acyltransferases; EC 2.3.1.91/sinapoylglucose-choline O-sinapoyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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