Document Detail


Targeted metabolomics analysis of Campylobacter coli VC167 reveals legionaminic acid derivatives as novel flagellar glycans.
MedLine Citation:
PMID:  17371878     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glycosylation of Campylobacter flagellin is required for the biogenesis of a functional flagella filament. Recently, we used a targeted metabolomics approach using mass spectrometry and NMR to identify changes in the metabolic profile of wild type and mutants in the flagellar glycosylation locus, characterize novel metabolites, and assign function to genes to define the pseudaminic acid biosynthetic pathway in Campylobacter jejuni 81-176 (McNally, D. J., Hui, J. P., Aubry, A. J., Mui, K. K., Guerry, P., Brisson, J. R., Logan, S. M., and Soo, E. C. (2006) J. Biol. Chem. 281, 18489-18498). In this study, we use a similar approach to further define the glycome and metabolomic complement of nucleotide-activated sugars in Campylobacter coli VC167. Herein we demonstrate that, in addition to CMP-pseudaminic acid, C. coli VC167 also produces two structurally distinct nucleotide-activated nonulosonate sugars that were observed as negative ions at m/z 637 and m/z 651 (CMP-315 and CMP-329). Hydrophilic interaction liquid chromatography-mass spectrometry yielded suitable amounts of the pure sugar nucleotides for NMR spectroscopy using a cold probe. Structural analysis in conjunction with molecular modeling identified the sugar moieties as acetamidino and N-methylacetimidoyl derivatives of legionaminic acid (Leg5Am7Ac and Leg5AmNMe7Ac). Targeted metabolomic analyses of isogenic mutants established a role for the ptmA-F genes and defined two new ptm genes in this locus as legionaminic acid biosynthetic enzymes. This is the first report of legionaminic acid in Campylobacter sp. and the first report of legionaminic acid derivatives as modifications on a protein.
Authors:
David J McNally; Annie J Aubry; Joseph P M Hui; Nam H Khieu; Dennis Whitfield; Cheryl P Ewing; Patricia Guerry; Jean-Robert Brisson; Susan M Logan; Evelyn C Soo
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-03-19
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  282     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-07     Completed Date:  2007-06-28     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14463-75     Citation Subset:  IM    
Affiliation:
National Research Council, Institute for Biological Sciences, Ottawa, Ontario, Canada.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EF141522
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MeSH Terms
Descriptor/Qualifier:
Biosynthetic Pathways
Campylobacter coli / genetics*,  metabolism
Chromatography, Liquid
Cyclic AMP / metabolism
Flagellin / chemistry,  metabolism*
Gene Expression Regulation, Bacterial*
Genes, Bacterial*
Glycosylation
Magnetic Resonance Spectroscopy
Models, Chemical
Models, Molecular
Molecular Sequence Data
Mutagenesis
Mutation
Polysaccharides / metabolism*
Sialic Acids / chemistry,  metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Grant Support
ID/Acronym/Agency:
R01 AI43559/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/5,7-diacetamido-8-O-acetyl-3,5,7,9-tetradeoxy-glycero-talo-nonulosonic acid; 0/Polysaccharides; 0/Sialic Acids; 12777-81-0/Flagellin; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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