Document Detail

Targeted metabolomics of Physaria fendleri, an industrial crop producing hydroxy fatty acids.
MedLine Citation:
PMID:  24443498     Owner:  NLM     Status:  Publisher    
Physaria fendleri (syn. lesquerella), is a Brassicaceae producing lesquerolic acid, a highly-valued hydroxy fatty acid that could be used for several industrial applications, such as cosmetics, lubricating greases, paints, plastics, and biofuels. Free of toxins, physaria is an attractive alternative to imported castor (Ricinus communis) oil, and is hence on the verge of commercialization. Gas chromatography-mass spectrometry analysis of fatty acid methyl esters revealed that lesquerolic acid was synthesized and accumulated in the embryos, reaching 60% (w/w) of the total fatty acids. The sequential extraction and characterization of biomass compounds divulged that physaria embryo metabolism switched from protein to fatty acid biosynthesis between 18 and 24 days post anthesis (DPA). In order to unravel the metabolic pathways involved in fatty acid synthesis, a targeted metabolomics study was conducted on physaria embryos at different stages of development. For this purpose, two novel high-throughput liquid chromatography tandem mass spectrometry methods were developed and validated to quantify sugars, sugar alcohols, and amino acids. Specificity was achieved using multiple reaction monitoring, and the limits of quantification were in the pmol-fmol range. The comparative metabolomic study underlined that: i) the majority of the metabolites accumulate in physaria embryos between 18 and 27 DPA; ii) the oxidative pentose-phosphate pathway, glycolysis, the tricarboxilic acid cycle and the anaplerotic pathway drain a substantial amount of carbon; iii) ribulose 1,5 bisphosphate is present, which specifically indicates that the Calvin cycle is occurring. The importance and the relevance of these findings regarding fatty acid synthesis were discussed.
Jean-Christophe Cocuron; Brooke Anderson; Alison Boyd; Ana Paula Alonso
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-18
Journal Detail:
Title:  Plant & cell physiology     Volume:  -     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-1-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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