Document Detail


Targeted knockdown of insulin-like growth factor binding protein-2 disrupts cardiovascular development in zebrafish embryos.
MedLine Citation:
PMID:  15618288     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
IGF binding protein-2 (IGFBP-2) is an evolutionarily conserved protein that binds IGFs and modulates their biological activities. Although the actions of IGFBP-2 have been well studied in vitro, we have a poor understanding of its in vivo functions, particularly during early development. Using the transparent zebrafish embryo as a model, we show that IGFBP-2 mRNA is expressed in lens epithelium and cranial boundary regions during early embryonic development and becomes localized to the liver by the completion of embryogenesis. Targeted knock-down of IGFBP-2 by antisense morpholino-modified oligonucleotides resulted in delayed development, reduced body growth, reduced IGF-I mRNA levels, and disruptions to cardiovascular development, including reduced blood cell number, reduced blood circulation, cardiac dysfunction, and brain ventricle edema. Detailed examination of vascular tissues, using a stable transgenic line of zebrafish expressing green fluorescent protein in vascular endothelial cells, revealed specific angiogenic (vessel sprouting) defects in IGFBP-2 knockdown embryos, with effects being localized in regions associated with IGFBP-2 mRNA expression. These findings suggest that IGFBP-2 is required for general embryonic development and growth and plays a local role in regulating vascular development in a model vertebrate organism.
Authors:
Antony W Wood; Peter J Schlueter; Cunming Duan
Related Documents :
8647318 - Growth hormone (gh) status regulates gh receptor and gh binding protein mrna in a tissu...
10082578 - Maturation of the myogenic program is induced by postmitotic expression of insulin-like...
16901968 - Epithelial-specific and stage-specific functions of insulin-like growth factor-i during...
14684598 - Insulin-like growth factor-induced transcriptional activity of the skeletal alpha-actin...
16232198 - Establishment of an immortalized cell line from a precancerous lesion of lung adenocarc...
7854358 - Cloning of a novel orphan receptor (gcnf) expressed during germ cell development.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2004-12-23
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  19     ISSN:  0888-8809     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-23     Completed Date:  2005-08-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1024-34     Citation Subset:  IM    
Affiliation:
Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Avenue, Ann Arbor, Michigan 48109, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cardiovascular Abnormalities / genetics
Cardiovascular System / embryology*
Down-Regulation
Embryo, Nonmammalian / abnormalities,  metabolism
Embryonic Development
Gene Expression / drug effects
Gene Targeting
Hematopoiesis / genetics,  physiology
Insulin-Like Growth Factor Binding Protein 2 / genetics,  physiology*
Insulin-Like Growth Factor I / genetics
Oligodeoxyribonucleotides, Antisense / genetics,  pharmacology
RNA, Messenger / analysis,  metabolism
Zebrafish / embryology*,  genetics
Chemical
Reg. No./Substance:
0/Insulin-Like Growth Factor Binding Protein 2; 0/Oligodeoxyribonucleotides, Antisense; 0/RNA, Messenger; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The zinc finger Ikaros transcription factor regulates pituitary growth hormone and prolactin gene ex...
Next Document:  Deletion of the RIIbeta-subunit of protein kinase A decreases body weight and increases energy expen...