Document Detail


Targeted glypican-3 gene transcription inhibited the proliferation of human hepatoma cells by specific short hairpin RNA.
MedLine Citation:
PMID:  23192642     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hepatocellular carcinoma (HCC) is a highly chemoresistant cancer with no effective systemic therapy. Despite of surgical or locoregional therapies, prognosis remains poor because of high tumor recurrence or progression, and currently, there are no well-established effective adjuvant therapies. Glypican-3 (GPC-3) is specifically overexpressed in hepatoma and perhaps is a valuable molecular target for HCC therapy. In this present study, the effect of silencing GPC-3 gene transcription on human HepG2 cell proliferation was investigated by constructing GPC-3 short hairpin RNA (shRNA) plasmid. After HepG2 cells were transfected with the most efficient shRNA, GPC-3 mRNA expression (90.4 %) was inhibited significantly and estimated by fluorescence quantitative reverse transcriptase-polymerase chain reaction, and the result was accordance with downregulation at the protein level. The percentage of the cell proliferation was down to 28.9 % in the shRNA group and 19.9 % in the shRNA plus sorafenib group. The cell cycles were arrested in the G1 phase (65.6 %) and the apoptosis rate was increasing (66.75 %) in the shRNA1 group with significant alteration compared with that in the negative-shRNA group. Specific shRNA might intervene effectively GPC-3 activation and inhibit tumor cell proliferation, suggesting that GPC-3 gene should be a potential molecular target for HCC therapy.
Authors:
Dandan Yu; Zhizhen Dong; Min Yao; Wei Wu; Meijuan Yan; Xiaodi Yan; Liwei Qiu; Jie Chen; Wenli Sai; Dengfu Yao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-29
Journal Detail:
Title:  Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine     Volume:  34     ISSN:  1423-0380     ISO Abbreviation:  Tumour Biol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-15     Completed Date:  2013-05-07     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  8409922     Medline TA:  Tumour Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  661-8     Citation Subset:  IM    
Affiliation:
Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, 20 West Temple Road, Nantong, 226001, Jiangsu Province, China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
Blotting, Western
Carcinoma, Hepatocellular / genetics,  metabolism,  pathology*
Cell Cycle
Cell Proliferation*
Glypicans / antagonists & inhibitors,  genetics*,  metabolism
Humans
Liver Neoplasms / genetics,  metabolism,  pathology*
RNA, Messenger / genetics
RNA, Small Interfering / genetics*
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Glypicans; 0/RNA, Messenger; 0/RNA, Small Interfering
Comments/Corrections

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