| Targeted expression of a synthetic codon optimized gene, encoding the spruce budworm antifreeze protein, leads to accumulation of antifreeze activity in the apoplasts of transgenic tobacco. | |
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MedLine Citation:
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PMID: 11574159 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A synthetic gene based on the primary sequence of the mature spruce budworm antifreeze protein (sbwAFP) was constructed by primer overlap extension. The amino acid codons were chosen to mimic those of a highly expressed tobacco nuclear gene. A DNA sequence encoding the amino-terminal leader sequence from the tobacco pathogen related protein 1b (PR), which targets the protein to the apoplastic space, was fused in frame to the synthetic sbwAFP gene. This fusion was placed downstream of the cauliflower mosaic virus 35S promoter and upstream of the nopaline synthase terminator in a T-DNA binary vector. Transgenic tobacco lines transcribing PR-sbwAFP were selected by RT-PCR. The apoplastic protein fractions of sbwAFP expressing tobacco lines exhibited enhanced antifreeze activity as demonstrated by the ability to inhibit ice re-crystallization and increased thermal hysteresis. |
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Authors:
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N Holmberg; J Farrés; J E Bailey; P T Kallio |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Gene Volume: 275 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2001-09-27 Completed Date: 2001-12-13 Revised Date: 2006-04-21 |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: Netherlands |
Other Details:
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Languages: eng Pagination: 115-24 Citation Subset: IM |
Affiliation:
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Unilever Research, Colworth House, Sharnbrook, Bedford MK44 1LQ, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological
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genetics Animals Antifreeze Proteins / genetics* Codon / genetics Freezing* Gene Expression Genes, Synthetic / genetics* Genetic Vectors / genetics Insect Proteins / genetics Plant Leaves / genetics Plants, Genetically Modified / genetics, growth & development RNA, Messenger / genetics, metabolism Tobacco / genetics*, growth & development Transcription, Genetic Transformation, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Antifreeze Proteins; 0/Codon; 0/Insect Proteins; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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