| Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks. | |
| | |
MedLine Citation:
|
PMID: 17159906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The Drosophila element Mos1 is a class II transposon, which moves by a 'cut-and-paste' mechanism and can be experimentally mobilized in the Caenorhabditis elegans germ line. Here, we triggered the excision of identified Mos1 insertions to create chromosomal breaks at given sites and further manipulate the broken loci. Double-strand break (DSB) repair could be achieved by gene conversion using a transgene containing sequences homologous to the broken chromosomal region as a repair template. Consequently, mutations engineered in the transgene could be copied to a specific locus at high frequency. This pathway was further characterized to develop an efficient tool--called MosTIC--to manipulate the C. elegans genome. Analysis of DSB repair during MosTIC experiments demonstrated that DSBs could also be sealed by end-joining in the germ line, independently from the evolutionarily conserved Ku80 and ligase IV factors. In conjunction with a publicly available Mos1 insertion library currently being generated, MosTIC will provide a general tool to customize the C. elegans genome. |
| | |
Authors:
|
Valérie Robert; Jean-Louis Bessereau |
Related Documents
:
|
1374516 - A branching process model of gene amplification following chromosome breakage. 3010046 - Identification of a dna sequence associated with plasmid integration in streptomyces co... 15007706 - Evolutionary implication of human endogenous retrovirus herv-h family. 11728156 - Potential role of transposable elements in the rapid reorganization of the fusarium oxy... 14762016 - Pria is essential for viability of the escherichia coli topoisomerase iv pare10(ts) mut... 10677316 - Evidence for heterogeneity in recombination in the human pseudoautosomal region: high r... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-12-07 |
Journal Detail:
|
Title: The EMBO journal Volume: 26 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 2007 Jan |
Date Detail:
|
Created Date: 2007-01-10 Completed Date: 2007-03-09 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 8208664 Medline TA: EMBO J Country: England |
Other Details:
|
Languages: eng Pagination: 170-83 Citation Subset: IM |
Affiliation:
|
ENS, Biologie cellulaire de la synapse, Paris, France; Inserm, U789, Paris, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Base Sequence Caenorhabditis elegans / genetics*, physiology Computational Biology DNA Breaks, Double-Stranded* DNA-Binding Proteins / genetics* Drosophila melanogaster / metabolism Genetic Techniques* Genome* Models, Genetic Molecular Sequence Data Mutagenesis Plasmids / metabolism Recombination, Genetic Transgenes* Transposases / genetics* |
| Chemical | |
Reg. No./Substance:
|
0/DNA-Binding Proteins; 0/mariner transposases; EC 2.7.7.-/Transposases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Structural and functional insights into the human Upf1 helicase core.
Next Document: Palmitoylation of CD95 facilitates formation of SDS-stable receptor aggregates that initiate apoptos...