Document Detail


Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks.
MedLine Citation:
PMID:  17159906     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Drosophila element Mos1 is a class II transposon, which moves by a 'cut-and-paste' mechanism and can be experimentally mobilized in the Caenorhabditis elegans germ line. Here, we triggered the excision of identified Mos1 insertions to create chromosomal breaks at given sites and further manipulate the broken loci. Double-strand break (DSB) repair could be achieved by gene conversion using a transgene containing sequences homologous to the broken chromosomal region as a repair template. Consequently, mutations engineered in the transgene could be copied to a specific locus at high frequency. This pathway was further characterized to develop an efficient tool--called MosTIC--to manipulate the C. elegans genome. Analysis of DSB repair during MosTIC experiments demonstrated that DSBs could also be sealed by end-joining in the germ line, independently from the evolutionarily conserved Ku80 and ligase IV factors. In conjunction with a publicly available Mos1 insertion library currently being generated, MosTIC will provide a general tool to customize the C. elegans genome.
Authors:
Valérie Robert; Jean-Louis Bessereau
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-12-07
Journal Detail:
Title:  The EMBO journal     Volume:  26     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-10     Completed Date:  2007-03-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  170-83     Citation Subset:  IM    
Affiliation:
ENS, Biologie cellulaire de la synapse, Paris, France; Inserm, U789, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Caenorhabditis elegans / genetics*,  physiology
Computational Biology
DNA Breaks, Double-Stranded*
DNA-Binding Proteins / genetics*
Drosophila melanogaster / metabolism
Genetic Techniques*
Genome*
Models, Genetic
Molecular Sequence Data
Mutagenesis
Plasmids / metabolism
Recombination, Genetic
Transgenes*
Transposases / genetics*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/mariner transposases; EC 2.7.7.-/Transposases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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