Document Detail

Targeted ablation of cardiac sympathetic neurons reduces resting, reflex and exercise-induced sympathetic activation in conscious rats.
MedLine Citation:
PMID:  19304949     Owner:  NLM     Status:  MEDLINE    
Cholera toxin B subunit conjugated to saporin (SAP, a ribosomal inactivating protein that binds to and inactivates ribosomes) was injected in both stellate ganglia to evaluate the physiological response to targeted ablation of cardiac sympathetic neurons. Resting cardiac sympathetic activity (cardiac sympathetic tonus), exercise-induced sympathetic activity (heart rate responses to graded exercise), and reflex sympathetic activity (heart rate responses to graded doses of sodium nitroprusside, SNP) were determined in 18 adult conscious Sprague-Dawley male rats. Rats were randomly divided into the following three groups (n = 6/group): 1) control (no injection), 2) bilateral stellate ganglia injection of unconjugated cholera toxin B (CTB), and 3) bilateral stellate ganglia injection of cholera toxin B conjugated to SAP (CTB-SAP). CTB-SAP rats, compared with control and CTB rats, had reduced cardiac sympathetic tonus and reduced heart rate responses to graded exercise and graded doses of SNP. Furthermore, the number of stained neurons in the stellate ganglia and spinal cord (segments T(1)-T(4)) was reduced in CTB-SAP rats. Thus CTB-SAP retrogradely transported from the stellate ganglia is effective at ablating cardiac sympathetic neurons and reducing resting, exercise, and reflex sympathetic activity. Additional studies are required to further characterize the physiological responses to this procedure as well as determine if this new approach is safe and efficacious for the treatment of conditions associated with excess sympathetic activity (e.g., autonomic dysreflexia, hypertension, heart failure, and ventricular arrhythmias).
Heidi L Lujan; Gurunanthan Palani; Ying Chen; Jean D Peduzzi; Stephen E Dicarlo
Related Documents :
15878799 - The effects of regular aerobic exercise in adolescent period on hippocampal neuron dens...
7637549 - Stimulation of myofibrillar protein synthesis in hindlimb suspended rats by resistance ...
9211179 - Progression of renal failure after subtotal nephrectomy in transgenic rats carrying an ...
15048889 - Delayed myelopathy induced by chronic compression in the rat spinal cord.
11041559 - Skeletal muscle hsp72 level during endurance training: influence of peripheral arterial...
7207199 - Thyroid hormones and lipolysis in physically trained rats.
15640329 - The timed walk test as a measure of severity and survival in idiopathic pulmonary fibro...
2764689 - Improving the validity of clinical exercise testing: the relationship between practice ...
16100159 - Effects of lipo-prostaglandin e1 on pulmonary hemodynamics and clinical outcomes in pat...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-03-20
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  296     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-04     Completed Date:  2009-06-19     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1305-11     Citation Subset:  IM    
Department of Physiology, Wayne State Univ. School of Medicine, 540 E. Canfield Ave., Detroit, MI 48201, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adrenergic Fibers / drug effects*,  metabolism,  pathology
Axonal Transport
Blood Pressure / drug effects
Cholera Toxin / administration & dosage,  metabolism,  pharmacology*
Dose-Response Relationship, Drug
Heart / innervation*
Heart Rate / drug effects
Nitroprusside / pharmacology
Physical Exertion*
Rats, Sprague-Dawley
Reflex / drug effects*
Ribosome Inactivating Proteins, Type 1 / administration & dosage,  metabolism,  pharmacology*
Stellate Ganglion / drug effects*,  metabolism,  pathology
Sympathectomy, Chemical*
Time Factors
Grant Support
Reg. No./Substance:
0/Ribosome Inactivating Proteins, Type 1; 0/cholera toxin B-saporin conjugate; 15078-28-1/Nitroprusside; 9012-63-9/Cholera Toxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Hypoxia during pregnancy in rats leads to early morphological changes of atherosclerosis in adult of...
Next Document:  Aspergillus alabamensis, a new clinically relevant species in the section Terrei.