Document Detail

Targeted therapy for Ewing's sarcoma: significance of heterogeneity.
MedLine Citation:
PMID:  20944159     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Survival in Ewing's sarcoma (ES) is limited. Experience with insulin-like growth factor targeting drugs, which require specific molecular tumour alterations, herald a major breakthrough. We screened for tumour heterogeneity within patients by DNA quantification. MATERIALS AND METHODS: DNA image cytometry (IC) was performed on 41 samples from 21 patients, evaluating if ploidy state remained constant over time and between different lesions within patients and the prognostic value of ploidy was assessed. RESULTS: DNA content varied over time and different ploidy states were found to coexist at a single timepoint. Non-diploid DNA content was associated with shorter overall survival (median, 19 vs. 84 months, p=0.047). CONCLUSION: We encountered a change and heterogeneity of ploidy state. This implies that screening for targets on a single tumour sample is insufficient and may lead to under- or overtreatment. The fact that non-diploid DNA content was associated with an adverse outcome confirms that this technique discriminates biologically different tumour clones.
A C M Van De Luijtgaarden; W T A Van Der Graaf; I Otte-Höller; H W B Schreuder; Y M H Versleijen-Jonkers; P J Slootweg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-10-14     Completed Date:  2010-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  3715-9     Citation Subset:  IM    
Department of Medical Oncology, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, the Netherlands.
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MeSH Terms
Bone Neoplasms / genetics*,  mortality
DNA, Neoplasm / genetics*
Drug Delivery Systems
Image Cytometry
Kaplan-Meiers Estimate
Sarcoma, Ewing's / genetics*,  mortality
Reg. No./Substance:
0/DNA, Neoplasm

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