| Target proteins of the cytosolic thioredoxin in Plasmodium falciparum. | |
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MedLine Citation:
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PMID: 20307687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The target proteins of a cytosolic Trx (PfTrx-1) in Plasmodium falciparum with Trx-affinity chromatography were examined. Based on the Trx protein reduction pathway, we generated a cysteine mutant of PfTrx-1, which captures the target protein as a mixed disulfide intermediate. A number of proteins were captured with PfTrx-1(C33S) immobilized on resin and were eluted by DTT treatment. The PfTrx-1(C33S) immobilized resin-captured proteins were trypsin-digested and analyzed on a liquid chromatography-mass spectrometry system. Analysis of the sequence data against databases assigned 20 proteins, four of which had been found previously in P. falciparum, with the remaining 16 being new targets. The potential Trx-target proteins included those in pathways such as the redox cycle, protein biosynthesis, energy metabolism and signal transduction. We captured 4 enzymes in the glycolysis pathway (hexokinase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycerate mutase and L-lactate dehydrogenase (LDH)) as Trx-targets, and we found that PfTrx-1 enhanced the activity of PfGAPDH and PfLDH. |
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Authors:
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Shin-ichiro Kawazu; Hitoshi Takemae; Kanako Komaki-Yasuda; Shigeyuki Kano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-20 |
Journal Detail:
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Title: Parasitology international Volume: 59 ISSN: 1873-0329 ISO Abbreviation: Parasitol. Int. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-04 Completed Date: 2010-07-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9708549 Medline TA: Parasitol Int Country: Netherlands |
Other Details:
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Languages: eng Pagination: 298-302 Citation Subset: IM |
Affiliation:
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National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan. skawazu@obihiro.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Affinity Cytosol / enzymology* Gene Expression Regulation* Glyceraldehyde-3-Phosphate Dehydrogenases / genetics, metabolism L-Lactate Dehydrogenase / genetics, metabolism Oxidation-Reduction Plasmodium falciparum / enzymology*, genetics, metabolism Protozoan Proteins / genetics, metabolism* Thioredoxins / genetics, metabolism* Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Protozoan Proteins; 52500-60-4/Thioredoxins; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases |
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