Document Detail

Tanshinone (Salviae miltiorrhizae extract) preparations attenuate aminoglycoside-induced free radical formation in vitro and ototoxicity in vivo.
MedLine Citation:
PMID:  12760856     Owner:  NLM     Status:  MEDLINE    
Antioxidant therapy protects against aminoglycoside-induced ototoxicity in animal models. A clinically suitable antioxidant must not affect the therapeutic efficacy of aminoglycosides or exhibit any side effects of its own. In addition, the treatment should be inexpensive and convenient in order to be implemented in developing countries where the use of aminoglycosides is most common. Standardized Salviae miltiorrhizae extracts (Danshen) are used clinically in China and contain diterpene quinones and phenolic acids with antioxidant properties. We combined in vitro and in vivo approaches to investigate the effect of a clinically approved injectable Danshen solution on aminoglycoside-induced free radical generation and ototoxicity. In vitro, Danshen inhibited gentamicin-dependent lipid peroxidation (formation of conjugated dienes from arachidonic acid), as well as the gentamicin-catalyzed formation of superoxide (in a lucigenin-based chemiluminescence assay) and hydroxyl radicals (oxidation of N,N-dimethyl-p-nitrosoaniline). Danshen extracts were then administered to adult CBA mice receiving concurrent treatment with kanamycin (700 mg/kg of body weight twice daily for 15 days). Auditory threshold shifts induced by kanamycin (approximately 50 dB) were significantly attenuated. Danshen did not reduce the levels in serum or antibacterial efficacy of kanamycin. These results suggest that herbal medications may be a significantly underexplored source of antidotes for aminoglycoside ototoxicity. Such traditional medicines are widely used in many developing countries and could become an easily accepted and inexpensive protective therapy.
Ai-Mei Wang; Su-Hua Sha; Wojciech Lesniak; Jochen Schacht
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  47     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-05-22     Completed Date:  2003-10-07     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1836-41     Citation Subset:  IM    
Kresge Hearing Research Institute, University of Michigan, Ann Arbor, Michigan 48109-0506, USA.
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MeSH Terms
Anti-Bacterial Agents / adverse effects*,  blood
Auditory Threshold / drug effects
Cochlea / drug effects,  pathology
Diterpenes, Abietane
Drug Interactions
Drugs, Chinese Herbal / pharmacology*
Evoked Potentials, Auditory, Brain Stem / drug effects
Free Radical Scavengers / pharmacology
Gentamicins / adverse effects*
Hearing Loss / chemically induced*,  metabolism,  prevention & control*
Hydroxyl Radical / metabolism
Kanamycin / adverse effects*,  blood
Lipid Peroxidation / drug effects
Mice, Inbred CBA
Oxidants / adverse effects,  metabolism
Phenanthrenes / pharmacology*
Superoxides / metabolism
Grant Support
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Diterpenes, Abietane; 0/Drugs, Chinese Herbal; 0/Free Radical Scavengers; 0/Gentamicins; 0/Oxidants; 0/Phenanthrenes; 11062-77-4/Superoxides; 3352-57-6/Hydroxyl Radical; 568-73-0/tanshinone; 59-01-8/Kanamycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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