Document Detail


Tanshinone IIA inhibits human breast cancer cells through increased Bax to Bcl-xL ratios.
MedLine Citation:
PMID:  18698495     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tanshinone IIA (C19H18O3) was extracted from danshen (Salviae miltiorrhizae radix). It has cytotoxic properties and induces apoptosis in many human cancer cells. The molecular mechanisms are poorly understood, therefore, in the present study, we aimed to elucidate its anticancer activity on human breast cancer MDA-MB-231 cells. The cytotoxic effects of tanshinone IIA on MDA-MB-231 cells were measured by MTT assay. The percentages of cells in different cell cycle phases were determined by flow cytometry. The protein expression of Bax and Bcl-2 was examined using Western blotting. The results showed that tanshinone IIA inhibits the proliferation of MDA-MB-231 cells in a dose- and time-dependent manner. Tanshinone IIA induces apoptosis in a dose-dependent manner and the percentage of cells in sub-G1 phase. It increases the protein expression of Bax but decreases the Bcl-2 expression in MDA-MB-231 cells. Our findings suggest that tanshinone IIA can inhibit the proliferation of MDA-MB-231 cells by active apoptosis. One of the mechanisms may be through up-regulating the expression of Bax but down-regulating Bcl-2 expression and then inducing apoptosis. In conclusion, tanshinone IIA has therapeutic potential in breast cancer patients.
Authors:
Chin-Cheng Su; Yi-Hsiang Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  22     ISSN:  1107-3756     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-13     Completed Date:  2008-10-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  357-61     Citation Subset:  IM    
Affiliation:
Division of General Surgery, Buddhist Tzu Chi General Hospital, Hualien 970, Taiwan. succ.maeva@msa.hinet.net
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Apoptosis / drug effects
Breast Neoplasms / metabolism*,  pathology
Caspase 8 / metabolism
Cell Line, Tumor
Humans
Phenanthrenes / pharmacology*
bcl-2-Associated X Protein / metabolism*
bcl-X Protein / metabolism*
Chemical
Reg. No./Substance:
0/Actins; 0/Phenanthrenes; 0/bcl-2-Associated X Protein; 0/bcl-X Protein; 568-73-0/tanshinone; EC 3.4.22.-/Caspase 8

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