Document Detail

Tanshinone IIA down-regulates the protein expression of ErbB-2 and up-regulates TNF-alpha in colon cancer cells in vitro and in vivo.
MedLine Citation:
PMID:  19020785     Owner:  NLM     Status:  MEDLINE    
Tanshinone IIA (Tan-IIA) was isolated from Salviae Miltiorrhizae Radix. Our previous studies showed that Tan-IIA induced apoptosis in human colon cancer colo 205 cells, but the molecular mechanisms of the effect of Tan-IIA on human colon cancer were not clearly elucidated. The protein expression of ErbB-2 was up-regulated and activated in human and experimental colon cancers. In the present study, the effects of Tan-IIA on the protein expression of ErbB-2 in colo 205 cells were investigated. In vitro, colo 205 cells were treated with various concentrations of Tan-IIA (1, 2 and 5 mug/ ml) for 24 h, and the protein expression of TNF-alpha, ErbB-2 and caspase-3 was assayed by Western blotting. For the in vivo studies, male SCID mice were xenografted with colo 205 cells, and from day 10, Tan IIA (20 mg/kg/day, dissolved in corn oil) was administered by oral feeding for 30 days. As a control, mice with xenografted tumors were separately treated with corn oil (0.1 ml/10 g body weight). Expression of TNF-alpha, ErbB-2 and caspase-3 proteins was measured by Western blot analysis. Our results showed that Tan-IIA down-regulated the protein expression of ErbB-2 and up-regulated TNF-alpha and caspase-3 in colo 205 cells in vitro. In a colo 205 xenograft model, treatment with Tan-IIA caused up-regulation of TNF-alpha, caspase-3 and down-regulation of ErbB-2 protein expression as compared to the controls. Based on these observations, one possible molecular mechanisms by which Tan-IIA inhibits the proliferation of colo 205 cells is through the down-regulation of ErbB-2 protein expression and the up-regulation of the protein expression of TNF-alpha and caspase-3.
Chin-Cheng Su; Yi-Hsiang Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  22     ISSN:  1107-3756     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-07-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  847-51     Citation Subset:  IM    
Division of General Surgery, Buddhist Tzu Chi General Hospital, Hualien City 970, Taiwan.
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MeSH Terms
Actins / drug effects,  metabolism
Antineoplastic Agents, Phytogenic / pharmacology
Caspase 3 / metabolism
Cell Line, Tumor
Cell Proliferation / drug effects
Colonic Neoplasms / metabolism*
Disease Models, Animal
Mice, SCID
Neoplasm Transplantation
Phenanthrenes / pharmacology*
Receptor, erbB-2 / metabolism*
Tumor Necrosis Factor-alpha / metabolism*
Up-Regulation / drug effects
Reg. No./Substance:
0/Actins; 0/Antineoplastic Agents, Phytogenic; 0/Phenanthrenes; 0/Tumor Necrosis Factor-alpha; 568-73-0/tanshinone; EC, erbB-2; EC 3.4.22.-/Caspase 3

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