| Tanshinone IIA down-regulates the protein expression of ErbB-2 and up-regulates TNF-alpha in colon cancer cells in vitro and in vivo. | |
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MedLine Citation:
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PMID: 19020785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tanshinone IIA (Tan-IIA) was isolated from Salviae Miltiorrhizae Radix. Our previous studies showed that Tan-IIA induced apoptosis in human colon cancer colo 205 cells, but the molecular mechanisms of the effect of Tan-IIA on human colon cancer were not clearly elucidated. The protein expression of ErbB-2 was up-regulated and activated in human and experimental colon cancers. In the present study, the effects of Tan-IIA on the protein expression of ErbB-2 in colo 205 cells were investigated. In vitro, colo 205 cells were treated with various concentrations of Tan-IIA (1, 2 and 5 mug/ ml) for 24 h, and the protein expression of TNF-alpha, ErbB-2 and caspase-3 was assayed by Western blotting. For the in vivo studies, male SCID mice were xenografted with colo 205 cells, and from day 10, Tan IIA (20 mg/kg/day, dissolved in corn oil) was administered by oral feeding for 30 days. As a control, mice with xenografted tumors were separately treated with corn oil (0.1 ml/10 g body weight). Expression of TNF-alpha, ErbB-2 and caspase-3 proteins was measured by Western blot analysis. Our results showed that Tan-IIA down-regulated the protein expression of ErbB-2 and up-regulated TNF-alpha and caspase-3 in colo 205 cells in vitro. In a colo 205 xenograft model, treatment with Tan-IIA caused up-regulation of TNF-alpha, caspase-3 and down-regulation of ErbB-2 protein expression as compared to the controls. Based on these observations, one possible molecular mechanisms by which Tan-IIA inhibits the proliferation of colo 205 cells is through the down-regulation of ErbB-2 protein expression and the up-regulation of the protein expression of TNF-alpha and caspase-3. |
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Authors:
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Chin-Cheng Su; Yi-Hsiang Lin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 22 ISSN: 1107-3756 ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-07-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 847-51 Citation Subset: IM |
Affiliation:
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Division of General Surgery, Buddhist Tzu Chi General Hospital, Hualien City 970, Taiwan. succ.maeva@msa.hinet.net |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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drug effects,
metabolism Animals Antineoplastic Agents, Phytogenic / pharmacology Caspase 3 / metabolism Cell Line, Tumor Cell Proliferation / drug effects Colonic Neoplasms / metabolism* Disease Models, Animal Down-Regulation Humans Male Mice Mice, SCID Neoplasm Transplantation Phenanthrenes / pharmacology* Receptor, erbB-2 / metabolism* Tumor Necrosis Factor-alpha / metabolism* Up-Regulation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Antineoplastic Agents, Phytogenic; 0/Phenanthrenes; 0/Tumor Necrosis Factor-alpha; 568-73-0/tanshinone; EC 2.7.10.1/Receptor, erbB-2; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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