| Tannic acid and thiocarbohydrazide as structural reinforcement agents in the preparation of rabbit knee articular cartilage for the scanning electron microscope. | |
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MedLine Citation:
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PMID: 10405825 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Samples from seven sectors of the rabbit knee articular cartilage were shaved and prepared for the scanning electron microscope using either tannic acid, thiocarbohydrazide or nothing (control). Surface morphology was found to be more typical to a given sector and less so to a specific preparation procedure. Rough areas were recorded from load-bearing sectors, while smooth areas appeared on load-free ones. However, fibrillations were discerned on control load-bearing sectors only, and pits and humps were never detected. Tannic acid and thiocarbohydrazide may have exerted their structural reinforcing effect on the tissue preservation by enhancing the binding of osmium tetroxide to it, possibly along with that of other soluble tissue constituents. |
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Authors:
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D Levanon; H Stein |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Histochemical journal Volume: 31 ISSN: 0018-2214 ISO Abbreviation: Histochem. J. Publication Date: 1999 Jan |
Date Detail:
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Created Date: 1999-08-18 Completed Date: 1999-08-18 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0163161 Medline TA: Histochem J Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 71-3 Citation Subset: IM |
Affiliation:
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Unit of Electron Microscopy, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cartilage, Articular / drug effects*, ultrastructure Femur / drug effects, ultrastructure Fixatives / pharmacology* Hydrazines / pharmacology* Hydrolyzable Tannins / pharmacology* Knee Joint / drug effects*, ultrastructure Microscopy, Electron, Scanning Rabbits Tibia / drug effects, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Fixatives; 0/Hydrazines; 0/Hydrolyzable Tannins; 2231-57-4/thiocarbohydrazide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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