| Tannic acid-iron alum reactions: stain of choice for macroscopic sections of brain to be embedded in plastic. | |
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MedLine Citation:
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PMID: 49948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As a macroscopic stain for gross brain sections to be embedded in plastic, tannic acid-iron alum is superior to the generally recommended LeMasurier's variation of the Berlin blue technique because of its greater permanency in plastic. However, as originally adopted for use with brain tissue by Mulligan, the intense black staining of gray matter is too dark for plastic embedded specimens. A modification of this method designed to overcome this difficulty is described. Staining procedure: Wash formalin-fixed brain slices overnight in running water. Wash in distilled water, 2 changes, 30 minutes each. Place slices individually in Mulligan's solution at a temperature of 60-65 C for 4 minutes. Rinse in ice water for 10 seconds. Mordant in 0.4% tannic acid in distilled water for 1 minute. Wash in running tap water for 1 minute. Develop in 0.08% ferric ammonium sulfate in distilled water until gray matter is light gray, about 10-15 seconds. Wash in lukewarm running water for 1 hour, then gently hand-rub whitish film from myelinated surfaces. Store briefly in 3% formalin or 25% glycerine if necessary depending on plastic embedding procedure to be followed. |
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Authors:
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R V Gregg |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Stain technology Volume: 50 ISSN: 0038-9153 ISO Abbreviation: Stain Technol Publication Date: 1975 Mar |
Date Detail:
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Created Date: 1975-09-13 Completed Date: 1975-09-13 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0404535 Medline TA: Stain Technol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 87-91 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aluminum* Brain / anatomy & histology* Histological Techniques / methods* Hydrolyzable Tannins* Iron* Microtomy Plastics Staining and Labeling / methods Sulfates* Tannins* |
| Chemical | |
Reg. No./Substance:
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0/Hydrolyzable Tannins; 0/Plastics; 0/Sulfates; 0/Tannins; 7429-90-5/Aluminum; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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