Document Detail


Tandem mass spectrometry reveals the quaternary organization of macromolecular assemblies.
MedLine Citation:
PMID:  16793517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The application of mass spectrometry (MS) to the study of progressively larger and more complex macromolecular assemblies is proving increasingly useful for structural biologists. The scope of this approach has recently been widened through the application of a tandem MS procedure. This two-step technique involves the selection of specific assemblies in the gas phase and inducing their dissociation through collisions with argon atoms. Here, we investigate the mechanism of this process and show that dissociation of subunits from a macromolecular assembly follows a sequential pathway, with the partitioning of charge between the dissociation products governed primarily by their relative surface areas. Using this basis of understanding, we highlight differences in the dissociation pathways of three related macromolecular assemblies and show how these are a direct consequence of changes in both local and global oligomeric organization.
Authors:
Justin L P Benesch; J Andrew Aquilina; Brandon T Ruotolo; Frank Sobott; Carol V Robinson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemistry & biology     Volume:  13     ISSN:  1074-5521     ISO Abbreviation:  Chem. Biol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-23     Completed Date:  2006-08-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  597-605     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Heat-Shock Proteins / chemistry*
Methanococcus
Models, Molecular
Multiprotein Complexes / chemistry
Protein Binding
Protein Structure, Quaternary
Protein Subunits / chemistry
Spectrometry, Mass, Electrospray Ionization / methods*
Triticum
alpha-Crystallin B Chain / chemistry*
Chemical
Reg. No./Substance:
0/Heat-Shock Proteins; 0/Multiprotein Complexes; 0/Protein Subunits; 0/alpha-Crystallin B Chain

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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