Document Detail


Tamoxifen-induced alterations in meiotic maturation and cytogenetic abnormalities in mouse oocytes and 1-cell zygotes.
MedLine Citation:
PMID:  11358325     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alterations in the rate of oocyte meiotic maturation (OM) and the timing of the metaphase-anaphase transition may predispose oocytes to premature centromere separation (PCS) and aneuploidy. Tamoxifen has the potential for perturbing the rate of OM since it can function as a calcium antagonist by binding to calmodulin and inhibiting the formation of a calcium-calmodulin complex which is needed for activating calmodulin-dependent cAMP phosphodiesterase and initiating OM. The objective of this study was to test the hypothesis that tamoxifen alters the rate of OM and predisposes oocytes to PCS and aneuploidy. Different does of tamoxifen were administered by oral gavage to female mice preovulation. Metaphase II oocyte and 1-cell zygote chromosomes were C-banded and cytogenetically analysed. Tamoxifen treatment resulted in a modest, but significant (p < 0.05), increase in oocytes with PCS. Similar frequencies of hyperploidy and oocytes with unpaired, single chromatids (SC) were found. Metaphase I, diploid and premature anaphase (PA) oocytes were not detected. Hyperploidy, polyploidy, PCS, PA and SC were not detected in zygotes. These data indicate that the levels of tamoxifen-induced PCS found in mouse oocytes did not predispose zygotes to aneuploidy. Tamoxifen did, however, reduce the proportion of females exhibiting oestrus.
Authors:
S N London; J B Mailhes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  9     ISSN:  0967-1994     ISO Abbreviation:  Zygote     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-18     Completed Date:  2002-03-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  England    
Other Details:
Languages:  eng     Pagination:  97-104     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, Louisiana State University Medical Center, Shreveport 71130, USA. JMAILH@Isumc.edu
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MeSH Terms
Descriptor/Qualifier:
Anaphase / drug effects
Aneuploidy*
Animals
Centromere / drug effects,  physiology,  ultrastructure
Cytogenetic Analysis
Female
Male
Meiosis / drug effects*
Metaphase / drug effects
Mice
Mice, Inbred ICR
Oocytes / cytology*,  drug effects*,  metabolism,  ultrastructure
Ovulation / drug effects
Polyploidy
Tamoxifen / pharmacology*
Zygote / cytology*,  drug effects*,  metabolism,  ultrastructure
Chemical
Reg. No./Substance:
10540-29-1/Tamoxifen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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