| TAM receptor knockout mice are susceptible to retinal autoimmune induction. | |
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MedLine Citation:
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PMID: 21467176 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: TAM receptors are expressed mainly by dendritic cells and macrophages in the immune system, and mice lacking TAM receptors develop systemic autoimmune diseases because of inefficient negative control of the cytokine signaling in those cells. This study aims to test the susceptibility of the TAM triple knockout (tko) mice to the retina-specific autoantigen to develop experimental autoimmune uveoretinitis (EAU). METHODS: TAM tko mice that were or were not immunized with interphotoreceptor retinoid-binding protein (IRBP) peptides were evaluated for retinal infiltration of the macrophages and CD3(+) T cells by immunohistochemistry, spontaneous activation of CD4(+) T cells, and memory T cells by flow cytometry and proliferation of IRBP-specific CD4(+) T cells by [(3)H]thymidine incorporation assay. Ocular inflammation induced by IRBP peptide immunization and specific T cell transfer were observed clinically by funduscopy and confirmed by histology. RESULTS: Tko mice were found to have less naive, but more activated, memory T cells, among which were exhibited high sensitivity to ocular IRBP autoantigens. Immunization with a low dose of IRBP and adoptive transfer of small numbers of IRBP-specific T cells from immunized tko mice caused the infiltration of lymphocytes, including CD3(+) T cells, into the tko retina. CONCLUSIONS: Mice without TAM receptor spontaneously develop IRBP-specific CD4(+) T cells and are more susceptible to retinal autoantigen immunization. This TAM knockout mouse line provides an animal model with which to study the role of antigen-presenting cells in the development of T cell-mediated uveitis. |
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Authors:
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Fei Ye; Qiutang Li; Yan Ke; Qingjun Lu; Lixia Han; Henry J Kaplan; Hui Shao; Qingxian Lu |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-06-16 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 52 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-06-17 Completed Date: 2011-09-01 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 4239-46 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, Kentucky 40202, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoantigens / immunology* Autoimmune Diseases / immunology*, metabolism, pathology Dendritic Cells / immunology Disease Models, Animal Disease Susceptibility / immunology* Eye Proteins / immunology Female Lymphocyte Activation / immunology Mice Mice, Knockout Retina / immunology*, pathology Retinal Diseases / immunology*, metabolism, pathology Retinol-Binding Proteins / immunology T-Lymphocytes / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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R01 EY019891-02/EY/NEI NIH HHS; R01-EY018830/EY/NEI NIH HHS; R01-EY01989/EY/NEI NIH HHS; RR017702/RR/NCRR NIH HHS; RR018733/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Autoantigens; 0/Eye Proteins; 0/Retinol-Binding Proteins; 0/interstitial retinol-binding protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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