| Talin concentrates to the midbody region during mammalian cell cytokinesis. | |
| | |
MedLine Citation:
|
PMID: 10544209 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In this study we investigated the cellular distribution of talin, a cytoskeletal protein, during mammalian cell cytokinesis. Immunohistochemical experiments on various carcinoma cell lines and mesenchyme-derived cells reveal that talin displays a cell cycle-dependent cellular localization. During metaphase, talin is located in the centromeric region of the chromosome, like the TD-60 protein and intrinsic centromere components detected by a CREST serum. From anaphase to telophase, talin is present in the cleavage furrow. As the cells progress to cytokinesis, when the furrow is complete, talin is concentrated in the midbody structures, as assessed by immunofluorescence and confirmed by Western blot experiments on purified midbodies. Double staining experiments reveal that alpha-tubulin, TD-60 protein, and talin co-localize in the midbodies. These results suggest that talin, in addition to its implication in focal adhesion organization and signaling, may play a critical role in cytokinesis. (J Histochem Cytochem 47:1357-1367, 1999) |
| | |
Authors:
|
A Bellissent-Waydelich; M T Vanier; C Albigès-Rizo; P Simon-Assmann |
Related Documents
:
|
15813749 - Requirement for microtubules and dynein motors in the earliest stages of peroxisome bio... 3084259 - A centriole-free drosophila cell line. a high voltage em study. 3173379 - The interaction of triethyl lead with tubulin and microtubules. 20581449 - Centrosomal chk2 in dna damage responses and cell cycle progression. 3653409 - Cytoskeletal protein synthesis and organization in cultured mouse osteoblastic cells. e... 12626509 - Filament formation of msf-a, a mammalian septin, in human mammary epithelial cells depe... 21108119 - Supercritical carbon dioxide extraction of ethyl p-methoxycinnamate from kaempferia gal... 12040059 - Analysis of cell lineage relationships in taste buds. 18422329 - Extremely low temperature fermentations of grape must by potato-supported yeast, strain... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 47 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 1999 Nov |
Date Detail:
|
Created Date: 1999-12-09 Completed Date: 1999-12-09 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1357-68 Citation Subset: IM |
Affiliation:
|
INSERM U381, Strasbourg, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenocarcinoma Animals Cell Cycle / physiology* Cell Division Cell Line Centromere / physiology, ultrastructure Chromosomes, Human / physiology, ultrastructure Colonic Neoplasms Humans Intestinal Mucosa / cytology, physiology Mammals Metaphase Rats Talin / analysis, physiology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Talin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: VEGF and vascular fusion: implications for normal and pathological vessels.
Next Document: Immunohistochemical evidence for the NO cGMP signaling pathway in respiratory ciliated epithelia of ...